Liu Yuehan, Liu Tingting, Niu Jingqi, Wang Siyu, Zhang Xuesong, Song Shuang, Wang Zhu
NHC Key Laboratory of Trace Element Nutrition, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2023 May;52(3):465-496. doi: 10.19813/j.cnki.weishengyanjiu.2023.03.022.
Using liquid chromatography-ion trap-time of flight-mass spectrometry to establish a soybean phospholipids(PL) analysis method, carry out comprehensive structural identification and quantitative analysis of soybean PL molecule species, and obtain soybean PL molecular composition and content data.
The PL profiles of 10 soybean varieties cultivated in northeast China were determined by a hydrophilic interaction liquid chromatography-ion trap-time of flight-mass spectrometry(HILIC-ESI-IT-TOF-MS) system. The hydrophilic interaction liquid chromatography realized class separation of PLs, and ion trap-time of flight-mass spectrometry realized the estimation of head group type, fatty acyl structure, and substituent position. The identified PL molecule species were quantified using accurate mass extraction and internal standard method.
A total of 101 PL molecular species from 11 classes were estimated in soybean seeds, including 20 phosphatidylcholines(PC), 15 phosphatidylethanolamines(PE), 17 phosphatidylinositols(PI), 12 phosphatidylglycerols(PG), 9 phosphatidic acids(PA), 6 phosphatidylserines(PS), 5 lysophosphatidylcholines(LPC), 5 lysophosphatidylethanolamines(LPE), 6 lysophosphatidylinositols(LPI), 4 lysophosphatidylglycerols(LPG) and 2 lysophosphatidicacids(LPA). The limits of detection for the target object were ≤ 60 ng/mL and R~2 were all >0.99. The total concentration of PL ranged from 6873.1 to 12 678.6 μg/g in detected soybeans. Generally, most of the detected soybean cultivars were great resources of PL, especially SN-29, SN-61, HN-40 and DN-690. The concentrations of PC and PE in the tested soybean were higher, followed by PI, PG, PA and PS. Among the lysophospholipids, the concentration of LPC was the highest, followed by LPE, LPI, LPG and LPA.
The soybean PL component analysis method established based on the HILIC-IT-TOF-MS system can achieve good separation of various types of PL, and can accurately characterize the head group type, fatty acyl structure and substituent position of PL molecule species. The molecular composition and content data of soybean PL were obtained.
采用液相色谱 - 离子阱 - 飞行时间质谱法建立大豆磷脂（PL）分析方法，对大豆PL分子种类进行全面的结构鉴定和定量分析，获取大豆PL分子组成及含量数据。
采用亲水作用液相色谱 - 离子阱 - 飞行时间质谱（HILIC - ESI - IT - TOF - MS）系统测定东北地区种植的10个大豆品种的PL谱图。亲水作用液相色谱实现了PL的类别分离，离子阱 - 飞行时间质谱实现了对头部基团类型、脂肪酰基结构和取代基位置的估算。采用精确质量提取和内标法对鉴定出的PL分子种类进行定量。
在大豆种子中总共估算出11类101种PL分子种类，包括20种磷脂酰胆碱（PC）、15种磷脂酰乙醇胺（PE）、17种磷脂酰肌醇（PI）、12种磷脂酰甘油（PG）、9种磷脂酸（PA）、6种磷脂酰丝氨酸（PS）、5种溶血磷脂酰胆碱（LPC）、5种溶血磷脂酰乙醇胺（LPE）、6种溶血磷脂酰肌醇（LPI）、4种溶血磷脂酰甘油（LPG）和2种溶血磷脂酸（LPA）。目标物的检测限≤60 ng/mL，R²均>0.99。在检测的大豆中，PL的总浓度范围为6873.1至12678.6 μg/g。一般来说，大多数检测到的大豆品种是PL的优质来源，尤其是SN - 29、SN - 61、HN - 40和DN - 690。受试大豆中PC和PE的浓度较高，其次是PI、PG、PA和PS。在溶血磷脂中，LPC的浓度最高，其次是LPE、LPI、LPG和LPA。
基于HILIC - IT - TOF - MS系统建立的大豆PL成分分析方法能够实现各类PL的良好分离，并能准确表征PL分子种类的头部基团类型、脂肪酰基结构和取代基位置。获得了大豆PL的分子组成及含量数据。
