Bugga Paramesha, Stoner Michael W, Manning Janet R, Mushala Bellina A S, Thapa Dharendra, Scott Iain
Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15261.
Center for Metabolism and Mitochondrial Medicine, University of Pittsburgh, Pittsburgh, PA 15261.
bioRxiv. 2023 Jul 21:2023.07.21.550091. doi: 10.1101/2023.07.21.550091.
GCN5L1, also known as BLOC1S1 and BLOS1, is a small intracellular protein involved in a number of key biological processes. Over the last decade, GCN5L1 has been implicated in the regulation of protein lysine acetylation, energy metabolism, endo-lysosomal function, and cellular immune pathways. An increasing number of published papers have used commercially-available reagents to interrogate GCN5L1 function. However, in many cases these reagents have not been rigorously validated, leading to potentially misleading results. In this report we tested several commercially-available antibodies for GCN5L1, and found that two-thirds of those available did not unambiguously detect the protein by western blot in cultured mouse cells or liver tissue. These data suggest that previously published studies which used these unverified antibodies to measure GCN5L1 protein abundance, in the absence of other independent methods of corroboration, should be interpreted with appropriate caution.
GCN5L1，也被称为BLOC1S1和BLOS1，是一种参与多个关键生物学过程的小细胞内蛋白。在过去十年中，GCN5L1一直与蛋白质赖氨酸乙酰化、能量代谢、内溶酶体功能和细胞免疫途径的调节有关。越来越多已发表的论文使用市售试剂来探究GCN5L1的功能。然而，在许多情况下，这些试剂并未经过严格验证，从而导致可能产生误导性的结果。在本报告中，我们测试了几种市售的针对GCN5L1的抗体，发现其中三分之二的抗体在培养的小鼠细胞或肝脏组织中通过蛋白质印迹法无法明确检测到该蛋白。这些数据表明，先前发表的使用这些未经验证的抗体来测量GCN5L1蛋白丰度的研究，在缺乏其他独立确证方法的情况下，应谨慎解读。
