Greenman J, Melville T H
Microbios. 1978;21(83):23-32.
Acid phosphatase activity was detected in Streptococcus mutans strain NCTC 10832, and both acid and alkaline phosphatase in strains 2M2 and K1R. In batch culture, activity was maximal by mid exponential phase for 2M2 and at the end of this phase for NCTC 10832. Alkaline, but not acid, phosphatase activity of 2M2 and K1R increased when the inorganic phosphate in the medium was low; this was considered due, at least partly, to inducible or derepressible enzymes. In continuous culture, acid phosphatase activity of NCTC 10832 varied with the sugar substrate. The activity was increased by cell disruption and the degree of this increase for cells grown on different sugars parallelled the amounts of extracellular, insoluble polysaccharide produced on those sugars. Activity was highest for glucose-grown whole cells and for sucrose-grown disrupted cells.
在变形链球菌NCTC 10832菌株中检测到酸性磷酸酶活性，在2M2和K1R菌株中同时检测到酸性和碱性磷酸酶活性。在分批培养中，2M2菌株在指数中期活性最高，NCTC 10832菌株在该阶段结束时活性最高。当培养基中的无机磷酸盐含量较低时，2M2和K1R菌株的碱性磷酸酶活性增加，而酸性磷酸酶活性未增加；这被认为至少部分是由于诱导型或去阻遏型酶所致。在连续培养中，NCTC 10832菌株的酸性磷酸酶活性随糖底物的变化而变化。细胞破碎会增加该活性，并且在不同糖类上生长的细胞的活性增加程度与在这些糖类上产生的细胞外不溶性多糖的量平行。葡萄糖生长的完整细胞和蔗糖生长的破碎细胞的活性最高。
