Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Department of Speech and Hearing Sciences, International University of Health and Welfare, and the Voice and Swallowing Center, Fukuoka Sanno Hospital, Fukuoka, Japan.
Laryngoscope. 2024 Jan;134(1):305-314. doi: 10.1002/lary.30918. Epub 2023 Jul 28.
To examine the sustained effects of oropharyngeal capsaicin stimulation on the regulation of swallowing, we recorded the swallowing-related nerve activities during continuous infusion of capsaicin solution into the oropharynx.
In 33 in situ perfused brainstem preparation of rats, we recorded the activities of the vagus, hypoglossal, and phrenic nerves during fictive swallowing. The interburst intervals (IBIs) of the swallowing-related nerves during sequential pharyngeal swallowing (sPSW) elicited by electrical stimulation of the superior laryngeal nerve (SLN) during concurrent capsaicin stimulation of 10, 1, and 0.1 μM (n = 28) were compared with those during oropharyngeal infusion of saline (control) (n = 5).
The IBIs during SLN-induced sPSW were reduced at 5 min after initiation of continuous infusion of 10 and 1 μM capsaicin solution. The IBIs showed significant decreases to -25.8 ± 6.9%, -25.9 ± 5.3, -18.3 ± 3.7, and -12.0 ± 1.6 at 30 min following 1 μM capsaicin stimulation at SLN stimulus conditions at 5 Hz of 1.2 times threshold, 10 Hz of 40 μA, 5 Hz of 60 μA, and 10 Hz of 60 μA, respectively. Continuous capsaicin stimulation of 0.1 μM solution did not show significant sustained effects.
Pharmacological stimulation of capsaicin could provide time-dependent effects on the likelihood of swallowing, particularly subserving sustained facilitation of swallowing reflex with appropriate concentration of capsaicin.
NA Laryngoscope, 134:305-314, 2024.
为了研究口咽部辣椒素刺激对吞咽调节的持续影响，我们在持续向口咽部输注辣椒素溶液的过程中记录吞咽相关神经的活动。
在 33 个原位灌流的大鼠脑干标本中，我们在电刺激喉上神经（SLN）诱发的模拟吞咽（fictive swallowing）过程中记录迷走神经、舌下神经和膈神经的活动。我们比较了在同时接受 10、1 和 0.1 μM 辣椒素刺激（n=28）的情况下，SLN 刺激期间连续咽（sPSW）诱发的吞咽相关神经的爆发间间隔（IBI）与在口咽部输注盐水（对照）（n=5）时的 IBI。
在连续输注 10 和 1 μM 辣椒素溶液 5 分钟后，SLN 诱导的 sPSW 的 IBI 降低。在 SLN 刺激条件下，在 5 Hz 时为 1.2 倍阈值、10 Hz 时为 40 μA、5 Hz 时为 60 μA 和 10 Hz 时为 60 μA，当用 1 μM 辣椒素刺激时，IBI 分别显著降低至-25.8±6.9%、-25.9±5.3%、-18.3±3.7%和-12.0±1.6%，持续 30 分钟。持续 0.1 μM 辣椒素溶液刺激没有显示出显著的持续效应。
辣椒素的药理学刺激可能对吞咽的可能性产生时间依赖性影响，特别是在适当浓度的辣椒素作用下，持续促进吞咽反射。
无。喉科学杂志，134:305-314，2024。
