Wad N
Ther Drug Monit. 1986;8(3):358-60. doi: 10.1097/00007691-198609000-00021.
The degradation of clonazepam (CZP) in serum during exposure to light can markedly decrease the concentration of the drug. Decay of CZP was studied after exposure to artificial UV light, sunlight, and room light. Serum was extracted with diethylether at pH 9.1. A reversed-phase (RP-8) column was used, with acetonitrile and water as the mobile phase. CZP was detected at 320 nm. The linearity of this high performance liquid chromatographic method is guaranteed to at least 700 nM. The recovery of CZP was 93%, and the day-to-day coefficients of variation were 7.7% (at 75.0 nM) and 6.0% (at 211.4 nM), respectively.
氯硝西泮（CZP）在光照下于血清中的降解会显著降低药物浓度。研究了氯硝西泮在人工紫外线、阳光和室内光照射后的降解情况。血清在pH 9.1条件下用乙醚萃取。使用反相（RP - 8）柱，以乙腈和水作为流动相。在320 nm处检测氯硝西泮。该高效液相色谱法的线性至少保证到700 nM。氯硝西泮的回收率为93%，日间变异系数分别为7.7%（75.0 nM时）和6.0%（211.4 nM时）。
