Ferraz Caline G, Ribeiro Paulo R, Verde Brenno V, Dos S Silva Rafael, do Carmo C Silva Maria, do Carmo Cristiano O, Bazioli Jaqueline M, Dos Santos Iago B F, Fill Taicia P, Sforca Mauricio L, Silva Franceli, da S Magaton Andréia, Soares Ana Cristina F
Centro de Ciências Exatas e Tecnológicas-CETEC, Universidade Federal do Recôncavo da Bahia, Rua Rui Barbosa, no710, 44.380-000, Cruz das Almas, Brazil.
Metabolomics Research Group, Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo s/n, 40170-115, Salvador, Brazil.
Chem Biodivers. 2023 Sep;20(9):e202300346. doi: 10.1002/cbdv.202300346. Epub 2023 Aug 14.
Pleurotus ostreatus is an edible fungus with high nutritional value that uses industrial and agricultural lignocellulosic residues as substrates for growth and reproduction. Understanding their growth metabolic dynamics on agro-industrial wastes would help to develop economically viable and eco-friendly biotechnological strategies for food production. Thus, we used UHPLC/MS/MS and GNPS as an innovative approach to investigate the chemical composition of two strains of P. ostreatus, coded as BH (Black Hirataki) and WH (White Hirataki), grown on sisal waste mixture (SW) supplemented with 20 % cocoa almond tegument (CAT) or 20 % of wheat bran (WB). Metabolite dereplication allowed the identification of 53 metabolites, which included glycerophospholipids, fatty acids, monoacylglycerols, steroids, carbohydrates, amino acids, and flavonoids. This is the first report of the identification of these compounds in P. ostreatus, except for the steroid ergosterol. Most of the metabolites described in this work possess potential biological activities, which support the nutraceutical properties of P. ostreatus. Thus, the results of this study provide essential leads to the understanding of white-rot fungi chemical plasticity aiming at developing alternative biotechnologies strategies for waste recycling.
平菇是一种具有高营养价值的食用菌，它以工农业木质纤维素残渣为生长和繁殖的底物。了解它们在工农业废弃物上的生长代谢动态，将有助于开发经济可行且环保的食品生产生物技术策略。因此，我们采用超高效液相色谱/串联质谱法（UHPLC/MS/MS）和全球天然产物社会分子网络（GNPS）作为一种创新方法，来研究两种平菇菌株（编码为BH（黑平茸）和WH（白平茸））在添加了20%可可杏仁皮（CAT）或20%麦麸（WB）的剑麻废料混合物（SW）上生长时的化学成分。代谢物去重复分析鉴定出了53种代谢物，包括甘油磷脂、脂肪酸、单酰甘油、类固醇、碳水化合物、氨基酸和黄酮类化合物。除了类固醇麦角固醇外，这是首次在平菇中鉴定出这些化合物的报告。这项工作中描述的大多数代谢物都具有潜在的生物活性，这支持了平菇的营养保健特性。因此，本研究结果为理解白腐真菌的化学可塑性提供了重要线索，旨在开发废弃物回收利用的替代生物技术策略。
