Department of Mechanical Engineering, University of Akron, Akron, OH 44325, USA.
Department of Biomedical Engineering, University of Akron, Akron, OH 44325, USA.
Biosensors (Basel). 2023 Jul 9;13(7):721. doi: 10.3390/bios13070721.
Rapid and accurate analysis of micro/nano bio-objects (e.g., cells, biomolecules) is crucial in clinical diagnostics and drug discovery. While a traditional resistive pulse sensor can provide multiple kinds of information (size, count, surface charge, etc.) about analytes, it has low throughput. We present a unique bipolar pulse-width, multiplexing-based resistive pulse sensor for high-throughput analysis of microparticles. Signal multiplexing is enabled by exposing the central electrode at different locations inside the parallel sensing channels. Together with two common electrodes, the central electrode encodes the electrical signal from each sensing channel, generating specific bipolar template waveforms with different pulse widths. Only one DC source is needed as input, and only one combined electrical output is collected. The combined signal can be demodulated using correlation analysis and a unique iterative cancellation scheme. The accuracy of particle counting and sizing was validated using mixtures of various sized microparticles. Results showed errors of 2.6% and 6.1% in sizing and counting, respectively. We further demonstrated its accuracy for cell analysis using HeLa cells.
快速准确地分析微/纳米生物目标（如细胞、生物分子）在临床诊断和药物发现中至关重要。虽然传统的电阻脉冲传感器可以提供关于分析物的多种信息（大小、数量、表面电荷等），但其吞吐量较低。我们提出了一种独特的基于双极脉冲宽度、复用的电阻脉冲传感器，用于微粒子的高通量分析。通过在平行传感通道内部的不同位置暴露中央电极，实现了信号复用。中央电极与两个公共电极一起，对每个传感通道的电信号进行编码，生成具有不同脉冲宽度的特定双极模板波形。仅需一个直流电源作为输入，仅需采集一个组合的电输出。组合信号可以使用相关分析和独特的迭代消除方案进行解调。使用不同大小的微粒子混合物验证了粒子计数和尺寸的准确性。结果表明，在尺寸和计数方面的误差分别为 2.6%和 6.1%。我们进一步使用 HeLa 细胞证明了其用于细胞分析的准确性。
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