Kim June Soo, Kwon Soon Yeol, Lee Jae Yong, Kim Seung Deok, Kim Da Ye, Kim Hyunjun, Jang Noah, Wang Jiajie, Han Maeum, Kong Seong Ho
School of Electronic and Electrical Engineering, Kyungpook National University, 41566, Daegu, Republic of Korea.
Lab Chip. 2023 Mar 28;23(7):1945-1953. doi: 10.1039/d2lc01064j.
A microfluidic resistive pulse sensing technique offers a simple method for detecting and analysing microparticles in various fields, yet it has challenges such as the noise during detection and low throughput as the signal obtained from a small single sensing aperture and particle position is nonuniform. This study presents a microfluidic chip with multiple detection gates in the main channel to enhance the throughput while maintaining a simple operational system. A hydrodynamic sheathless particle focusing on a detection gate by modulation of the channel structure and measurement circuit with a reference gate to minimize the noise during detection is used for detecting resistive pulses. The proposed microfluidic chip can analyse the physical properties of 200 nm polystyrene particles and exosomes from MDA-MB-231 with high sensitivity with an error of <10% and high-throughput screening of more than 200 000 exosomes per seconds. The proposed microfluidic chip can analyse the physical properties with high sensitivity, so that it can be potentially used for exosome detection in biological and clinical applications.
微流控电阻脉冲传感技术为检测和分析各个领域中的微粒提供了一种简单的方法，然而它也面临着一些挑战，比如检测过程中的噪声以及通量较低，这是因为从小的单个传感孔径获得的信号以及粒子位置是不均匀的。本研究提出了一种在主通道中具有多个检测门的微流控芯片，以在保持操作系统简单的同时提高通量。通过调节通道结构和使用带有参考门的测量电路，使流体动力无鞘粒子聚焦在检测门上，以最小化检测过程中的噪声，用于检测电阻脉冲。所提出的微流控芯片能够以小于10%的误差高灵敏度地分析200 nm聚苯乙烯颗粒和MDA-MB-231细胞来源外泌体的物理性质，并且每秒能够对超过200,000个外泌体进行高通量筛选。所提出的微流控芯片能够高灵敏度地分析物理性质，因此它有可能用于生物和临床应用中的外泌体检测。
