Sergeevichev David, Vasiliyeva Maria, Kuznetsova Elena, Chelobanov Boris
NMRC Named after Academician E.N. Meshalkin of the Ministry of Health of the Russian Federation, Novosibirsk 630055, Russia.
Vorozhtsov Institute of Organic Chemistry SB RAS, Novosibirsk 630090, Russia.
Biomimetics (Basel). 2023 Jul 17;8(3):315. doi: 10.3390/biomimetics8030315.
Widely used storage methods, including freezing or chemical modification, preserve the sterility of biological tissues but degrade the mechanical properties of materials used to make heart valve prostheses. Therefore, wet storage remains the most optimal option for biomaterials. Three biocidal solutions (an antibiotic mixture, an octanediol-phenoxyethanol complex solution, and a glycerol-ethanol mixture) were studied for the storage of native and decellularized porcine aorta and pulmonary trunk. Subsequent mechanical testing and microstructural analysis showed a slight increase in the tensile strength of native and decellularized aorta in the longitudinal direction. Pulmonary trunk elongation increased 1.3-1.6 times in the longitudinal direction after decellularization only. The microstructures of the tested specimens showed no differences before and after wet storage. Thus, two months of wet storage of native and decellularized porcine aorta and pulmonary trunks does not significantly affect the strength and elastic properties of the material. The wet storage protocol using alcohol solutions of glycerol or octanediol-phenoxyethanol mixture may be intended for further fabrication of extracellular matrix for tissue-engineered biological heart valve prostheses.
广泛使用的储存方法，包括冷冻或化学改性，可保持生物组织的无菌性，但会降低用于制造心脏瓣膜假体的材料的机械性能。因此，湿储存仍然是生物材料最理想的选择。研究了三种杀菌溶液（抗生素混合物、辛二醇 - 苯氧乙醇复合溶液和甘油 - 乙醇混合物）用于储存天然和脱细胞猪主动脉及肺动脉干。随后的力学测试和微观结构分析表明，天然和脱细胞主动脉在纵向方向的拉伸强度略有增加。仅脱细胞后，肺动脉干在纵向方向的伸长增加了1.3 - 1.6倍。测试标本的微观结构在湿储存前后没有差异。因此，天然和脱细胞猪主动脉及肺动脉干两个月的湿储存不会显著影响材料的强度和弹性性能。使用甘油或辛二醇 - 苯氧乙醇混合物的酒精溶液的湿储存方案可能适用于进一步制造用于组织工程生物心脏瓣膜假体的细胞外基质。
