Pan Jia-Jun, Ye Chao, Li Qin, Chen Shi-Song, Ning Xiao-Ping, Yu Qi, Li Bai-Ling, Liu Xiao-Hong, Xu Zhi-Yun
Department of Cardiovascular Surgery, Changhai Hospital, Naval Medical University Shanghai, China.
Department of Cardiothoracic Surgery, The 902nd Hospital of PLA Joint Service Support Force Bengbu, Anhui, China.
Am J Transl Res. 2025 May 15;17(5):3368-3379. doi: 10.62347/OVKI5914. eCollection 2025.
To evaluate the effects of 75% ethanol on the disinfection efficacy, biocompatibility, extracellular matrix (ECM) integrity, and mechanical properties of swim bladders from , a promising biomaterial for cardiovascular applications.
Swim bladders were immersed in 75% ethanol for 30, 60, or 120 minutes, followed by phosphate-buffered saline (PBS) rinsing. Disinfection efficacy was assessed using bacterial cultures; cytotoxicity and blood compatibility were evaluated using CCK-8 assays and hemolysis tests. The decellularization efficiency, ECM integrity, and mechanical properties were analyzed through histological staining, DNA quantification, and uniaxial tensile test.
75% ethanol effectively disinfected the swim bladder with minimal cytotoxicity and good hemocompatibility. Histological staining demonstrated preservation of collagen fibers, elastin, and glycosaminoglycans (GAGs), indicating minimal impact on the ECM structure. The uniaxial tensile test revealed that ethanol immersion caused tissue dehydration and increased stiffness, but these effects were reversible after PBS rinsing, with mechanical properties returning to baseline. DNA content and tissue thickness normalized following PBS washing, suggesting that ethanol did not impair decellularization.
75% ethanol is an effective disinfectant for swim bladder biomaterials, preserving biocompatibility, ECM structure, and mechanical properties after rinsing. These findings support the use of swim bladder-derived scaffolds in cardiovascular tissue engineering applications.
评估75%乙醇对一种有望用于心血管应用的生物材料——鱼鳔的消毒效果、生物相容性、细胞外基质（ECM）完整性及力学性能的影响。
将鱼鳔浸入75%乙醇中30、60或120分钟，随后用磷酸盐缓冲盐水（PBS）冲洗。使用细菌培养评估消毒效果；使用CCK-8测定法和溶血试验评估细胞毒性和血液相容性。通过组织学染色、DNA定量和单轴拉伸试验分析去细胞化效率、ECM完整性和力学性能。
75%乙醇能有效消毒鱼鳔，细胞毒性最小且血液相容性良好。组织学染色显示胶原纤维、弹性蛋白和糖胺聚糖（GAGs）得以保留，表明对ECM结构影响极小。单轴拉伸试验表明，乙醇浸泡导致组织脱水并增加硬度，但PBS冲洗后这些影响是可逆的，力学性能恢复至基线水平。PBS冲洗后DNA含量和组织厚度恢复正常，表明乙醇未损害去细胞化过程。
75%乙醇是鱼鳔生物材料的有效消毒剂，冲洗后能保留生物相容性、ECM结构和力学性能。这些发现支持在心血管组织工程应用中使用鱼鳔衍生支架。
