Ali Aarif, Rehman Muneeb U, Mushtaq Saima, Ahmad Sheikh Bilal, Khan Altaf, Karan Anik, Bashir Wani Amir, Ganie Showkat Ahmad, Mir Manzoor Ur Rahman
Department of Clinical Biochemistry, School of Biological Sciences, University of Kashmir, Srinagar 190006, India.
Division of Veterinary Biochemistry, Faculty of Veterinary Sciences & Animal Husbandry, SKUAST-Kashmir, Alusteng, Shuhama 190006, India.
Curr Issues Mol Biol. 2023 Jun 26;45(7):5317-5346. doi: 10.3390/cimb45070338.
Subclinical mastitis (SCM) is a predominant form of mastitis wherein major visible signs of disease are absent. The present study aimed to determine acute phase proteins (APPs) like ferritin, C-reactive protein (CRP), and microalbumin (Malb) in 135 composite milk and serum samples of healthy ( = 25) and SCM ( = 110) cows. As bovine mastitis is an inflammatory disease, the present study also aimed at finding novel anti-inflammatory compounds from natural sources by repurposing approach using computational studies. The findings of the present study revealed substantial elevation ( < 0.001) in milk SCC and an increase in ferritin, CRP, and Malb ( < 0.001) in milk and sera of the SCM group as compared to healthy animals. Receiver operating characteristics of milk SCC, milk, and serum APPs unraveled statistically substantial alteration ( < 0.001). Further, SCC was correlated with milk APPs ferritin (r = 0.26 **, < 0.002), CRP (r = 0.19 *, < 0.02), and Malb (r = 0.21 *, < 0.01). Additionally, milk SCC was correlated with serum ferritin (r = 0.28 **, < 0.001), CRP (r = 0.16, > 0.05), and Malb (r = 0.16, > 0.05). The findings of molecular docking revealed that Chaetoglobosin U was the most effective molecule that showed the highest binding affinity (kcal/mol) of -10.1 and -8.5 against ferritin and albumin. The present study concluded that the estimation of cow-side tests, SCC, and APPs in milk/serum is suitable to detect SCM and screening herd community. Furthermore, Chaetoglobosin U could be developed as a promising anti-inflammatory inhibitor; however, further studies are required to validate these findings.
亚临床型乳腺炎（SCM）是乳腺炎的一种主要形式，其中不存在明显的主要疾病体征。本研究旨在测定135份健康奶牛（n = 25）和患亚临床型乳腺炎奶牛（n = 110）的混合乳样和血清样本中的急性期蛋白（APPs），如铁蛋白、C反应蛋白（CRP）和微量白蛋白（Malb）。由于牛乳腺炎是一种炎症性疾病，本研究还旨在通过利用计算研究的重新利用方法，从天然来源中寻找新型抗炎化合物。本研究结果显示，与健康动物相比，亚临床型乳腺炎组奶牛的乳中体细胞计数（SCC）显著升高（P < 0.001），乳和血清中的铁蛋白、CRP和Malb也有所增加（P < 0.001）。乳SCC、乳和血清APPs的受试者工作特征曲线显示出具有统计学意义的显著变化（P < 0.001）。此外，SCC与乳APPs铁蛋白（r = 0.26 **，P < 0.002）、CRP（r = 0.19 *，P < 0.02）和Malb（r = 0.21 *，P < 0.01）相关。此外，乳SCC与血清铁蛋白（r = 0.28 **，P < 0.001）、CRP（r = 0.16，P > 0.05）和Malb（r = 0.16，P > 0.05）相关。分子对接结果显示，Chaetoglobosin U是最有效的分子，对铁蛋白和白蛋白表现出最高的结合亲和力（kcal/mol），分别为-10.1和-8.5。本研究得出结论，在奶牛场检测乳/血清中的SCC和APPs，适用于检测亚临床型乳腺炎和筛查畜群群体。此外，Chaetoglobosin U有望开发成为一种抗炎抑制剂；然而，需要进一步研究来验证这些发现。
