Bingol Ozakpinar Ozlem, Dastan Havva, Gurboga Merve, Sayin Fatih Serdar, Ozsavci Derya, Caliskan Salihi Elif
Department of Biochemistry, Faculty of Pharmacy, Marmara University, 34854 Istanbul, Turkey.
Department of Biochemistry, Health Sciences Institute, Marmara University, 34865 Istanbul, Turkey.
Gels. 2023 Jul 10;9(7):561. doi: 10.3390/gels9070561.
Colorectal cancer (CRC) is the fourth most commonly diagnosed cancer and the third leading cause of cancer-related deaths worldwide. A substantial body of literature supports the crucial role of vitamin D (VD) in the etiology, progression, prognosis, and treatment of cancer. Recent clinical studies have found an inverse correlation between CRC incidence and serum VD levels. However, the low water solubility of VD and its anticarcinogenic activity at supraphysiological plasma levels, which causes hypercalcemia, required carrier systems. Carbon-based nanomaterials are excellent eco-friendly candidates, with exceptional chemical resistance, efficient mechanical properties, and negligible weight. Furthermore, composite aerogels manufactured from these nanomaterials have gained interest due to their extensive surface areas and porous structures, which make them suitable for delivering drugs. Our research aimed to study the development of composite aerogels loaded with VD by utilizing carbon nanofibers (CNFs) in an aerogel matrix provided to colon cancer cells. For this purpose, Aero1 as a drug delivery system was first prepared and characterized using XRD, FTIR, and SEM methods. Biochemical methods were employed to evaluate the antiproliferative, apoptotic, and anti-migratory effects on colon cancer cells. FTIR and XRD measurements confirmed the production of aerogels. SEM analysis revealed that aerogels have a non-uniform surface. The findings showed that aerogels can effectively deliver VD to the colon cancer cells, while also inhibiting cancer cell proliferation and migration. This research suggests that the Aero1 drug delivery system could be a valuable tool in the fight against colon cancer and other health issues.
结直肠癌（CRC）是全球第四大常见诊断癌症，也是癌症相关死亡的第三大主要原因。大量文献支持维生素D（VD）在癌症的病因、进展、预后和治疗中起关键作用。最近的临床研究发现，结直肠癌发病率与血清VD水平呈负相关。然而，VD的低水溶性及其在超生理血浆水平下的抗癌活性会导致高钙血症，因此需要载体系统。碳基纳米材料是优秀的环保候选材料，具有出色的耐化学性、高效的机械性能和可忽略不计的重量。此外，由这些纳米材料制造的复合气凝胶因其广泛的表面积和多孔结构而受到关注，这使其适合用于药物递送。我们的研究旨在通过在气凝胶基质中利用碳纳米纤维（CNF）来研究负载VD的复合气凝胶的开发，并将其提供给结肠癌细胞。为此，首先制备了作为药物递送系统的Aero1，并使用XRD、FTIR和SEM方法对其进行了表征。采用生化方法评估其对结肠癌细胞的抗增殖、凋亡和抗迁移作用。FTIR和XRD测量证实了气凝胶的产生。SEM分析表明气凝胶表面不均匀。研究结果表明，气凝胶可以有效地将VD递送至结肠癌细胞，同时还能抑制癌细胞的增殖和迁移。这项研究表明，Aero1药物递送系统可能是对抗结肠癌和其他健康问题的有价值工具。
