Tsai Milton Hongli, Megat Abdul Wahab Rohaya, Zainal Ariffin Shahrul Hisham, Azmi Fazren, Yazid Farinawati
Discipline of Orthodontics, Department of Family Oral Health, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia.
Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Malaysia.
Gels. 2023 Jul 10;9(7):562. doi: 10.3390/gels9070562.
The challenges of using VEGF to promote osteoblastic differentiation include a short half-life and a narrow therapeutic window. A carrier system combining hydrogel and liposomes may improve the therapeutic efficacy of VEGF for bone regeneration. This study aimed to investigate the effects of delivery of VEGF via liposomal hydrogel on the osteogenesis of MG-63 cells. Liposomal hydrogel scaffold was fabricated and then characterized in terms of the morphological and chemical properties using FESEM and FTIR. In 2.5D analysis, the MG-63 cells were cultured on liposomal hydrogel + VEGF as the test group. The osteogenic effects of VEGF were compared with the control groups, i.e., hydrogel without liposomes + VEGF, osteogenic medium (OM) supplemented with a bolus of VEGF, and OM without VEGF. Cell morphology, viability, and differentiation and mineralization potential were investigated using FESEM, MTT assay, ALP activity, and Alizarin red staining. The characterization of scaffold showed no significant differences in the morphological and chemical properties between hydrogel with and without liposomes ( > 0.05). The final 2.5D culture demonstrated that cell proliferation, differentiation, and mineralization were significantly enhanced in the liposomal hydrogel + VEGF group compared with the control groups ( < 0.05). In conclusion, liposomal hydrogel can be used to deliver VEGF in a sustained manner in order to enhance the osteogenesis of MG-63 cells.
使用血管内皮生长因子（VEGF）促进成骨细胞分化面临的挑战包括半衰期短和治疗窗口窄。水凝胶与脂质体相结合的载体系统可能会提高VEGF促进骨再生的治疗效果。本研究旨在探讨通过脂质体水凝胶递送VEGF对MG-63细胞成骨作用的影响。制备脂质体水凝胶支架，然后使用场发射扫描电子显微镜（FESEM）和傅里叶变换红外光谱（FTIR）对其形态和化学性质进行表征。在2.5D分析中，将MG-63细胞培养在脂质体水凝胶+VEGF上作为试验组。将VEGF的成骨作用与对照组进行比较，对照组包括无脂质体的水凝胶+VEGF、补充一次大剂量VEGF的成骨培养基（OM）以及无VEGF的OM。使用FESEM、MTT法、碱性磷酸酶（ALP）活性检测和茜素红染色来研究细胞形态、活力、分化和矿化潜力。支架表征显示，有脂质体和无脂质体的水凝胶在形态和化学性质上无显著差异（P>0.05）。最终的2.5D培养结果表明，与对照组相比，脂质体水凝胶+VEGF组的细胞增殖、分化和矿化显著增强（P<0.05）。总之，脂质体水凝胶可用于持续递送VEGF，以增强MG-63细胞的成骨作用。
