Chemical Engineering Faculty, Sahand University of Technology, Tabriz, 51335-1996, Iran.
Stem Cell and Tissue Engineering Research Laboratory, Sahand University of Technology, Tabriz, 51335-1996, Iran.
J Nanobiotechnology. 2022 Jun 28;20(1):310. doi: 10.1186/s12951-022-01511-9.
Hydrogels based on organic/inorganic composites have been at the center of attention for the fabrication of engineered bone constructs. The establishment of a straightforward 3D microenvironment is critical to maintaining cell-to-cell interaction and cellular function, leading to appropriate regeneration. Ionic cross-linkers, Ca, Ba, and Sr, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.
Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups. Human MG-63 osteoblasts inside Sr cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation. PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth. Transplantation of Sr cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.
Sr cross-linked Alg-nHA-Col hydrogel can promote functionality and dynamic growth of osteoblasts.
基于有机/无机复合材料的水凝胶一直是工程化骨构建体制造的研究重点。建立一个简单的 3D 微环境对于维持细胞间相互作用和细胞功能至关重要，从而促进适当的再生。本研究使用离子交联剂 Ca、Ba 和 Sr 制备藻酸钠-纳米羟基磷灰石-胶原（Alg-nHA-Col）微球，并在体外和体内条件下，在第 21 天检测了人成骨细胞的成骨性能。
水凝胶的物理化学性质表明，与其他组相比，用 Sr 交联的微球具有更低的溶胀性、增强的稳定性和机械强度。Sr 交联微球内的人 MG-63 成骨细胞表现出增强的活力和成骨能力，表现为矿化和与骨形成相关的相关蛋白增加。Wnt（无翅型整合位点）信号通路的 PCR（聚合酶链反应）阵列分析表明，Sr 交联微球适当诱导了人成骨细胞中的各种信号转导通路，从而促进成骨活性和动态生长。将载有人成骨细胞的 Sr 交联微球移植到大鼠颅骨临界尺寸缺损模型中，通过 micro-CT 和组织学检查分析加速了骨形成。
Sr 交联的 Alg-nHA-Col 水凝胶可以促进成骨细胞的功能和动态生长。
