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昆虫蛋白饲料的基因鉴定与溯源

Genetic Identification and Traceability of Insect Meals.

作者信息

Moulistanos Aristotelis, Karaiskou Nikoleta, Gkagkavouzis Konstantinos, Minoudi Styliani, Drosopoulou Elena, Ioannidou Chrysanthi, Panteli Nikolas, Zografou Stella, Karaouglanis Damianos, Kotouzas Dimitrios, Kontodimas Dimitrios, Antonopoulou Efthimia, Triantafyllidis Alexandros

机构信息

Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

Genomics and Epigenomics Translational Research (GENeTres), Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, 57001 Thessaloniki, Greece.

出版信息

Insects. 2023 Jul 5;14(7):610. doi: 10.3390/insects14070610.

Abstract

Insects have been proposed as a rich alternative source of protein for the partial or total replacement of fishmeal in aquaculture. For maximum safety and effectiveness of insect meals, control of the quality composition of these products is considered mandatory. The aim of this study was the genetic analysis of the composition of commercially available insect meals at the species level. Commercially available , and individuals, as well as nine insect meals produced from these species, were analyzed. The genetic identification of insects at the species level was based on a COI fragment, and analysis of the insect meals' composition was performed with the processes of cloning and colony PCR. Genetic analysis indicated that the commercially available larvae morphologically identified as belonged to the species . In the commercially available insect meals, no other animal species was identified beyond the expected one. However, in the insect meal produced for research purposes, fungal growth was detected. The used methodology, herein, allows for the qualitative genetic identification of insect meals and could be included in the methods of traceability of products containing insects and other animal species.

摘要

昆虫已被提议作为一种丰富的蛋白质替代来源,用于部分或完全替代水产养殖中的鱼粉。为了确保昆虫饲料的最大安全性和有效性,对这些产品的质量成分进行控制被认为是必不可少的。本研究的目的是在物种水平上对市售昆虫饲料的成分进行遗传分析。分析了市售的[具体昆虫种类1]、[具体昆虫种类2]和[具体昆虫种类3]个体,以及由这些物种生产的九种昆虫饲料。昆虫在物种水平上的遗传鉴定基于一个细胞色素氧化酶亚基I(COI)片段,并且通过克隆和菌落聚合酶链式反应(PCR)过程对昆虫饲料的成分进行分析。遗传分析表明,形态上鉴定为[具体昆虫种类1]的市售幼虫属于[实际所属物种1]。在市售昆虫饲料中,除了预期的物种外,未鉴定出其他动物物种。然而,在用于研究目的生产的昆虫饲料中,检测到了真菌生长。本文所使用的方法能够对昆虫饲料进行定性遗传鉴定,并且可以纳入含有昆虫和其他动物物种的产品可追溯性方法中。

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