Ye Yu, Zhan Xufang, Wang Kai, Zhong Jingya, Liao Fanglei, Chen Wenrong, Guo Weidong
College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
School of Marine Sciences, Ningbo University, Ningbo 315211, China.
J Fungi (Basel). 2023 Jul 24;9(7):779. doi: 10.3390/jof9070779.
Blueberry ( spp.) rhizosphere microorganisms can significantly increase the absorption area and improve the efficiency of rhizospheric nutrient uptake. However, there has been little research on blueberry rhizosphere microorganisms, especially those that can complement root function deficiency. In this study, we analyzed the rhizosphere fungi of 'O'Neal,' 'Sharpblue,' and 'Premier' blueberry cultivars and found that 'Premier' blueberries showed strong growth potential and relatively high root regulation ability. The dominant symbiotic fungus was correlated with the strong growth of 'Premier' and was directionally screened and isolated based on conserved gene structures and COG function analysis. This fungus was reinoculated onto the roots of 'Gulfcoast' and 'Star' blueberry cultivars. promoted the growth of blueberries and improved their ability to resist stress and grow under adverse conditions, as indicated by maintained or increased chlorophyll content under such conditions. Further analyses showed that has certain functional characteristics such as the ability to dissolve iron in its insoluble form and then release it, to fix nitrogen, and to inhibit nitrification in soil. Thus, it effectively doubled the soil nitrogen content and increased the soluble iron content in soil by 50%. This investigation indicates inoculation as an approach to increase blueberry stress tolerance and complete their root nutrition deficiency.
蓝莓（spp.）根际微生物能显著增加吸收面积并提高根际养分吸收效率。然而，关于蓝莓根际微生物的研究较少，尤其是那些能弥补根系功能缺陷的微生物。在本研究中，我们分析了‘奥尼尔’‘夏普蓝’和‘总理’蓝莓品种的根际真菌，发现‘总理’蓝莓表现出较强的生长潜力和相对较高的根系调控能力。优势共生真菌与‘总理’蓝莓的旺盛生长相关，并基于保守基因结构和COG功能分析进行了定向筛选和分离。将这种真菌重新接种到‘海湾海岸’和‘明星’蓝莓品种的根系上。在不利条件下，叶绿素含量维持或增加，表明其促进了蓝莓生长并提高了它们抵御胁迫和生长的能力。进一步分析表明，该真菌具有某些功能特性，如能够溶解不溶性铁并释放出来、固氮以及抑制土壤中的硝化作用。因此，它有效地使土壤氮含量翻倍，并使土壤中可溶性铁含量增加了50%。这项研究表明接种该真菌是提高蓝莓胁迫耐受性和弥补其根系营养缺陷的一种方法。
