Popov Marek, Kubeš Jan, Vachová Pavla, Hnilička František, Zemanová Veronika, Česká Jana, Praus Lukáš, Lhotská Marie, Kudrna Jiří, Tunklová Barbora, Štengl Karel, Krucký Jiří, Turnovec Tomáš
Department of Botany and Plant Physiology, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha-Suchdol, Czech Republic.
Department of Agroenvironmental Chemistry and Plant Nutrition, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha-Suchdol, Czech Republic.
Toxics. 2023 Jun 29;11(7):568. doi: 10.3390/toxics11070568.
Experimental spinach plants grown in soil with (5, 10 and 20 ppm) arsenic (As) contamination were sampled in 21 days after As(V) contamination. Levels of As in spinach samples (from 0.31 ± 0.06 µg g to 302.69 ± 11.83 µg g) were higher in roots and lower in leaves, which indicates a low ability of spinach to translocate As into leaves. Species of arsenic, As(III) and As(V), were represented in favor of the As (III) specie in contaminated variants, suggesting enzymatic arsenate reduction. In relation to predominant As accumulation in roots, changes in malondialdehyde levels were observed mainly in roots, where they decreased significantly with growing As contamination (from 11.97 ± 0.54 µg g in control to 2.35 ± 0.43 µg g in 20 ppm As). Higher values in roots than in leaves were observed in the case of 5-methylcytosine (5-mC). Despite that, a change in 5-mC by As contamination was further deepened in leaves (from 0.20 to 14.10%). In roots of spinach, expression of the CDC25 gene increased by the highest As contamination compared to the control. In the case of total phenolic content, total flavonoid content, total phenolic acids content and total antioxidant capacity were higher levels in leaves in all values, unlike the roots.
在添加了（5、10和20 ppm）砷（As）的污染土壤中种植的实验菠菜植株，在砷（V）污染21天后进行采样。菠菜样品中的砷含量（从0.31±0.06 µg/g到302.69±11.83 µg/g）根部较高，叶片较低，这表明菠菜将砷转运到叶片中的能力较低。在受污染的变体中，砷的种类As(III)和As(V)以As(III)为主，表明存在酶促砷酸盐还原。关于根部主要积累砷的情况，丙二醛水平的变化主要在根部观察到，随着砷污染的增加，根部丙二醛水平显著下降（从对照中的11.97±0.54 µg/g降至20 ppm砷处理下的2.35±0.43 µg/g）。在5-甲基胞嘧啶（5-mC）方面，根部的值高于叶片。尽管如此，砷污染导致叶片中5-mC的变化进一步加深（从0.20%增至14.10%）。与对照相比，在菠菜根部，CDC25基因的表达在最高砷污染水平下增加。在总酚含量、总黄酮含量、总酚酸含量和总抗氧化能力方面，所有数值下叶片中的水平均高于根部。
