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新鲜三文鱼中腐败细菌的探索性研究:以真空和调味料作为天然防腐剂延长保质期

An Exploratory Study on Spoilage Bacteria and in Fresh Salmon: Extending Shelf-Life Using Vacuum and Seasonings as Natural Preservatives.

作者信息

Lemos Maria-Leonor, Prata Joana C, Rodrigues Inês C, Martins-Costa Sofia, Archer Bernardo, Machado Jorge, Dilão Rui, Vaz-Pires Paulo, Martins da Costa Paulo

机构信息

ICBAS-Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.

CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal.

出版信息

Vet Sci. 2023 Jun 29;10(7):423. doi: 10.3390/vetsci10070423.

Abstract

A growing population increases the demand for food, but short shelf-lives and microbial hazards reduce supply and increase food waste. Fresh fish is highly perishable and may be consumed raw, such as salmon in sushi. This work aims to identify strategies to improve the shelf-life and safety of fresh salmon, using available methods (i.e., vacuum) and exploring the use of natural preservatives (i.e., seasonings). Vacuum packaging and good hygiene practices (which reduce initial flora) extended shelf-life up to 20 days. was dominant in vacuum packaging conditions and showed potential for inhibiting . For natural preservatives, required higher inhibitory concentrations in vitro when compared to the 10 spoilage bacteria isolated from fresh salmon fillets, presenting a minimum inhibitory concentration (MIC) of 0.13% for oregano essential oil (OEO), 10% for lemon juice, 50 mg mL for garlic powder, and >10% for NaCl. A good bacteriostatic and bactericidal effect was observed for a mixture containing 5% NaCl, 0.002% OEO, 2.5% lemon juice, and 0.08 mg mL garlic powder. Finally, using the salmon medium showed an adequate correlation with the commercial culture medium.

摘要

人口增长增加了对食物的需求,但短保质期和微生物危害减少了供应并增加了食物浪费。鲜鱼极易腐烂,可能会生食,如寿司中的三文鱼。这项工作旨在确定改善鲜三文鱼保质期和安全性的策略,采用现有方法(即真空包装)并探索使用天然防腐剂(即调味料)。真空包装和良好的卫生习惯(可减少初始菌群)将保质期延长至20天。在真空包装条件下占主导地位,并显示出抑制的潜力。对于天然防腐剂,与从新鲜三文鱼片分离出的10种腐败细菌相比,在体外需要更高的抑制浓度,牛至精油(OEO)的最低抑菌浓度(MIC)为0.13%,柠檬汁为10%,大蒜粉为50 mg/mL,氯化钠为大于10%。对于含有5%氯化钠、0.002% OEO、2.5%柠檬汁和0.08 mg/mL大蒜粉的混合物,观察到良好的抑菌和杀菌效果。最后,使用三文鱼培养基与商业培养基显示出充分的相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fa/10383938/c62465f26f45/vetsci-10-00423-g0A1.jpg

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