School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea.
College of Life Sciences, Anhui Agricultural University, Hefei 230036, People's Republic of China.
J Nat Prod. 2023 Aug 25;86(8):1891-1900. doi: 10.1021/acs.jnatprod.3c00193. Epub 2023 Jul 28.
spp. are well-known symbiotic microorganisms that produce antimicrobial metabolites against various pathogens. We isolated actinomycetes from the body surface of the termite and identified it as BYF101 based on 16S rRNA phylogenetic analysis. Chemical analysis of the cultures of termite-associated BYF101 via HR-MS and GNPS analyses enabled the isolation and identification of 20 metabolites, including the unreported obscurolide-type metabolites (-). The chemical structures of unreported compounds (-) were elucidated using HR-ESI-MS and 1D and 2D NMR analysis, and their absolute configurations were determined via chemical reactions followed by the application of competing enantioselective acylation (CEA) and computational methods for ECD and DP4+ probability calculation. The isolated compounds (-) were tested to determine their antifungal activity against two human fungal pathogens, and Among the compounds tested, indole-3-carboxylic acid () displayed antifungal activity against , with an MIC value of 12 μg/mL.
种是众所周知的共生微生物，它们产生针对各种病原体的抗菌代谢物。我们从白蚁的体表分离出放线菌，并根据 16S rRNA 系统发育分析将其鉴定为 BYF101。通过 HR-MS 和 GNPS 分析对与白蚁相关的 BYF101 培养物进行化学分析，能够分离和鉴定出 20 种代谢产物，包括未报道的 obscurolide 型代谢产物（-）。使用 HR-ESI-MS 以及 1D 和 2D NMR 分析阐明了未报道化合物（-）的化学结构，并通过化学反应确定了它们的绝对构型，然后应用竞争性对映选择性酰化（CEA）和计算方法进行 ECD 和 DP4+概率计算。对分离得到的化合物（-）进行了抗真菌活性测试，以确定它们对两种人类真菌病原体 和 的抑制作用。在所测试的化合物中，吲哚-3-羧酸（）对 具有抗真菌活性，MIC 值为 12 μg/mL。
Zotero 插件