Department of Anatomy, College of Korean Medicine, Kyung Hee University, 26, Kyunghee dae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.
J Ethnopharmacol. 2024 Jan 10;318(Pt B):116953. doi: 10.1016/j.jep.2023.116953. Epub 2023 Jul 26.
Phyllostachys nigra (PN) is an herbal medicine that originates from the inner bark of Phyllostachys nigra Munro var. henosis Stapf or Phyllostachys bambusoides Siebold et Zuccarini. It has long been used to relieve fever and to treat diarrhea and inflammation. PN has been shown to possess inhibitory effects on pneumonia, intestinal inflammation, tumors, and fatigue. However, its potential efficacy in the treatment of atopic dermatitis (AD) has not been extensively studied or reported.
The objective of this research was to investigate the impact of PN on HaCaT and HMC-1 cells, as well as its potential in an experimental model of AD induced by 1-chloro-2,4-dinitrobenzene (DNCB).
We analyzed the anti-inflammatory efficacy of PN in HaCaT cells and HMC-1 cells using ELISA and PCR, and investigated invasion of inflammatory cell, change of dermis and epidermis, and the SCORAD index in AD-like mice model. We also measured the MAPK signaling pathway using the dorsal tissue of mice.
Our results show that PN reduced the expressions of TARC, GM-CSF, TNF-α, MCP-1, and IL-6 in vitro. PN also decreased the SCORAD index, thickening of epidermis and dermis, and inhibited the invasions of mast cells and eosinophils as well as CD4 T and CD8 T cells. Furthermore, PN suppressed the level of IgE and IL-6, and also inhibited the MAPK phosphorylation in the dorsal skin.
These results demonstrate that PN could be an effective alternative medicine for allergic inflammatory disease.
刚竹（PN）是一种草药，来源于刚竹或苦竹的内树皮。它长期以来被用于退热、治疗腹泻和炎症。PN 已被证明对肺炎、肠道炎症、肿瘤和疲劳具有抑制作用。然而，其在治疗特应性皮炎（AD）方面的潜在疗效尚未得到广泛研究或报道。
本研究旨在探讨 PN 对 HaCaT 和 HMC-1 细胞的影响及其在 1-氯-2,4-二硝基苯（DNCB）诱导的 AD 实验模型中的潜在作用。
我们使用 ELISA 和 PCR 分析了 PN 在 HaCaT 细胞和 HMC-1 细胞中的抗炎作用，并在 AD 样小鼠模型中研究了炎症细胞浸润、真皮和表皮变化以及 SCORAD 指数。我们还使用小鼠背部组织测量了 MAPK 信号通路。
结果表明，PN 降低了体外 TARC、GM-CSF、TNF-α、MCP-1 和 IL-6 的表达。PN 还降低了 SCORAD 指数、表皮和真皮增厚，抑制了肥大细胞和嗜酸性粒细胞以及 CD4 T 和 CD8 T 细胞的浸润。此外，PN 抑制了背部皮肤中 IgE 和 IL-6 的水平，并抑制了 MAPK 磷酸化。
这些结果表明，PN 可能是治疗过敏性炎症性疾病的有效替代药物。
