School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
Bioresour Technol. 2023 Oct;386:129576. doi: 10.1016/j.biortech.2023.129576. Epub 2023 Jul 26.
Regulating heavy metal resistance genes (HMRGs) was an effective method for heavy metal resistant bacteria (HMRB) to cope with heavy metal stress during dairy manure composting. This research aimed to investigate heavy metal detoxification mediated by shell powder (SP) in composting and the response of HMRB and HMRGs to changes in heavy metal bioavailability during composting. Research showed that SP additive reduced the bioavailability of Zu, Cu, and Mn by 10.64%, 13.90% and 14.14%, respectively. SP increased the composition percentage of humic acid (HA) in humus (HS) by 8%. SP enhanced the resistance of Actinobacteria to heavy metals and improved the regulation of HMRGs. Correlation analysis demonstrated that the bioavailability of heavy metals was positively correlated with most HMRGs. HA was significantly negatively correlated with the bioavailability of Zn, Cu and Mn. Therefore, SP additive could be a novel strategy for heavy metals detoxification during composting.
调控重金属抗性基因（HMRGs）是重金属抗性细菌（HMRB）在奶牛粪便堆肥过程中应对重金属胁迫的一种有效方法。本研究旨在探讨壳粉（SP）在堆肥过程中对重金属解毒的作用，以及 HMRB 和 HMRGs 对堆肥过程中重金属生物有效性变化的响应。研究表明，SP 添加剂分别降低了 Zn、Cu 和 Mn 的生物有效性 10.64%、13.90%和 14.14%。SP 增加了腐殖质（HS）中腐殖酸（HA）的组成百分比 8%。SP 增强了放线菌对重金属的抗性，并改善了 HMRGs 的调控。相关性分析表明，重金属的生物有效性与大多数 HMRGs 呈正相关。HA 与 Zn、Cu 和 Mn 的生物有效性呈显著负相关。因此，SP 添加剂可能是堆肥过程中重金属解毒的一种新策略。
