School of Basic Medical Science, Nanchang University, Nanchang 330031, China.
Key Laboratory of Molecular Biology and Genetic Engineering of Jiangxi Province, School of Life Science, Nanchang University, Nanchang 330031, China.
Gene. 2023 Oct 20;883:147671. doi: 10.1016/j.gene.2023.147671. Epub 2023 Jul 26.
Nonspecific lipid transfer proteins (nsLTPs) promote the intermembrane transportation of phospholipids, fatty acids, and steroids, and play diverse roles in various biological processes. However, the potential roles of the rice nsLTPs have not been well elucidated yet. Here, the functions of OsLTPL18 were analyzed using CRISPR/Cas9 strategy and cytological analysis. The osltpl18 (osltpl18-1, osltpl18-2, and osltpl18-3) seeds were thinner, and 1000-grain weight and grain thickness of osltpl18 plants were decreased obviously, compared to the ZH11. Meanwhile, the results of germination assay and 1 % TTC staining showed that vigor of osltpl18 seeds decreased significantly. Furthermore, the results of scanning electron microscopy (SEM) revealed that the cell width of spikelet hull in osltpl18 lines was significantly reduced than that in WT, as well as cell number in grain-width direction. Finally, we found that co-expressed genes were enriched in glucan biosynthesis, protein transporter activity, serine-type endopeptidase inhibitor activity, and nutrient reservoir activity. In this study, we discussed that OsLTPL18 might have coordinating functions in regulation of grain weight and germination in rice.
非特异性脂质转移蛋白（nsLTPs）促进磷脂、脂肪酸和类固醇的膜间转运，并在各种生物过程中发挥多种作用。然而，水稻 nsLTPs 的潜在作用尚未得到很好的阐明。在这里，我们使用 CRISPR/Cas9 策略和细胞学分析来分析 OsLTPL18 的功能。与 ZH11 相比，osltpl18（osltpl18-1、osltpl18-2 和 osltpl18-3）种子更薄，千粒重和粒厚明显降低。同时，发芽试验和 1%TTC 染色的结果表明，osltpl18 种子活力明显下降。此外，扫描电子显微镜（SEM）的结果表明，osltpl18 系小穗颖壳的细胞宽度明显小于 WT，粒宽方向的细胞数量也减少。最后，我们发现共表达基因富集在葡聚糖生物合成、蛋白质转运活性、丝氨酸内肽酶抑制剂活性和营养库活性。在这项研究中，我们讨论了 OsLTPL18 可能在调节水稻粒重和萌发方面具有协调功能。
