TCAF2 与免疫微环境相关，促进神经胶质瘤的发病机制，并损害其预后。
TCAF2 is associated with the immune microenvironment, promotes pathogenesis, and impairs prognosis in glioma.
机构信息
Department of Critical Care Medicine, Xuzhou Central Hospital, Xuzhou Clinical School of Nanjing Medical University, Xuzhou, Jiangsu 221009, China.
Department of Pathology, Xuzhou Central Hospital, Xuzhou Clinical School of Nanjing Medical University, Xuzhou, Jiangsu 221009, China.
出版信息
Gene. 2023 Oct 20;883:147667. doi: 10.1016/j.gene.2023.147667. Epub 2023 Jul 26.
PURPOSE
Glioma is the most common primary intracranial tumor and exhibits rapid growth and aggressiveness. TRPM8 channel-associated factor 2 (TCAF2), located in cell junctions and the plasma membrane, plays a key role in the pathogeneses of several cancers in humans. However, the role of TCAF2 in glioma has been elusive.
METHODS
A combination of bioinformatic analysis using The Cancer Genome Atlas database and biological experiments, including 5-ethynyl-2'-deoxyuridine, transwell, and immunohistochemistry assays and xenotransplantation, was performed to analyze the expression level of TCAF2 and to mechanistically explore the relationship of TCAF2 with malignancy, prognosis, and the immune microenvironment in glioma.
RESULTS
TCAF2 was upregulated in glioma, and its expression level correlated with tumor grade and clinical outcome. The role of TCAF2 in promoting glioma malignancy was characterized through in vitro and in vivo experiments. Additionally, we observed that TCAF2 can modulate the metabolic pathways and immune microenvironment.
CONCLUSION
TCAF2 acts as an oncogene and may serve as a therapeutic target and prognostic marker in glioma.
目的
神经胶质瘤是最常见的原发性颅内肿瘤，具有快速生长和侵袭性。位于细胞连接和质膜上的瞬时受体电位阳离子通道亚家族 M8 关联因子 2（TCAF2）在人类的几种癌症的发病机制中起着关键作用。然而，TCAF2 在神经胶质瘤中的作用仍不清楚。
方法
使用癌症基因组图谱数据库进行生物信息学分析，并结合包括 5-乙炔基-2'-脱氧尿苷、Transwell、免疫组织化学检测和异种移植在内的生物学实验，分析 TCAF2 的表达水平，并从机制上探讨 TCAF2 与神经胶质瘤恶性程度、预后和免疫微环境的关系。
结果
TCAF2 在神经胶质瘤中上调，其表达水平与肿瘤分级和临床预后相关。通过体外和体内实验证实了 TCAF2 促进神经胶质瘤恶性程度的作用。此外，我们观察到 TCAF2 可以调节代谢途径和免疫微环境。
结论
TCAF2 作为一种癌基因，可能在神经胶质瘤中作为治疗靶点和预后标志物。
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