Li Jianbo, Guo Wei, Zhao Jinna, Meng Huijing, Yang Yanfei, Zheng Guangshun, Yuan Weijie
Experimental Centre of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, China.
National State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.
Antioxidants (Basel). 2023 Jun 24;12(7):1339. doi: 10.3390/antiox12071339.
Drought stress is one of the major environmental factors severely restricting plant development and productivity. B, which is an economically important tree species, is highly tolerant to drought conditions, but the underlying molecular regulatory mechanisms remain relatively unknown. In this study, seedlings underwent a drought treatment (water withheld for 0, 3, 7, and 12 days), after which they were re-watered for 5 days. Physiological indices were measured and a transcriptome sequencing analysis was performed to reveal drought response-related regulatory mechanisms. In comparison to the control, the drought treatment caused a significant increase in antioxidant enzyme activities, with levels rising up to seven times, and relative electrical conductivity from 14.5% to 78.4%, but the relative water content decreased from 88.3% to 23.4%; these indices recovered somewhat after the 5-day re-watering period. The RNA sequencing analysis identified 9126 differentially expressed genes (DEGs), which were primarily involved with abscisic acid responses, and mitogen-activated protein kinase signaling. These DEGs included 483 (5.29%) transcription factor genes from 53 families, including , , and . A co-expression network analysis was conducted and three important modules were analyzed to identify hub genes, one of which () was examined to clarify its function. The AtruNAC36 protein was localized to the nucleus and had a C-terminal transactivation domain. Moreover, it bounded specifically to the NACRS element. The overexpression of in resulted in increased drought tolerance by enhancing antioxidant enzyme activities. These findings provide important insights into the transcriptional regulation mediating the response to drought. Furthermore, may be relevant for breeding forest trees resistant to drought stress.
干旱胁迫是严重限制植物生长发育和生产力的主要环境因素之一。B是一种具有重要经济价值的树种，对干旱条件具有高度耐受性，但其潜在的分子调控机制仍相对未知。在本研究中，幼苗经历了干旱处理（停水0、3、7和12天），之后再浇水5天。测量了生理指标并进行了转录组测序分析，以揭示与干旱响应相关的调控机制。与对照相比，干旱处理导致抗氧化酶活性显著增加，水平上升至7倍，相对电导率从14.5%升至78.4%，但相对含水量从88.3%降至23.4%；这些指标在5天的再浇水期后有所恢复。RNA测序分析鉴定出9126个差异表达基因（DEG），主要涉及脱落酸应答和丝裂原活化蛋白激酶信号传导。这些DEG包括来自53个家族的483个（5.29%）转录因子基因，包括 （此处原文缺失具体家族名称）、 （此处原文缺失具体家族名称）和 （此处原文缺失具体家族名称）。进行了共表达网络分析，并分析了三个重要模块以鉴定枢纽基因，其中一个（此处原文缺失具体基因名称）被研究以阐明其功能。AtruNAC36蛋白定位于细胞核，具有C端反式激活结构域。此外，它特异性地结合NACRS元件。在 （此处原文缺失具体植物名称）中过表达 （此处原文缺失具体基因名称）通过增强抗氧化酶活性导致耐旱性增加。这些发现为介导 （此处原文缺失具体植物名称）对干旱响应的转录调控提供了重要见解。此外， （此处原文缺失具体基因名称）可能与培育抗干旱胁迫的林木有关。
