Chakraborty Payal, Orvos Hajnalka, Hermesz Edit
Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, P.O. Box 533, H-6701 Szeged, Hungary.
Department of Pharmaceutical Technology, JIS University, 81, Nilgunj Road, Kolkata 700109, India.
Antioxidants (Basel). 2023 Jun 30;12(7):1370. doi: 10.3390/antiox12071370.
The increased rate of twinning has pointed out newer challenges in clinical practices related to gestational complications, intrauterine growth restriction, perinatal mortality, and comorbidities. As a twin pregnancy progresses, the increased demand for oxygen supply can easily disrupt the redox homeostasis balance and further impose a greater challenge for the developing fetuses. A substantial birth-weight difference acts as an indicator of a deficit in oxygenation or blood flow to one of the fetuses, which might be related to a low bioavailable nitric oxide level. Therefore, in this study, we focused on networks involved in the adjustment of oxygen supply, like the activation of inducible and endothelial nitric oxide synthase (NOS3) along with free radical and lipid peroxide formation in mature twin pairs with high birth-weight differences. The selected parameters were followed by immunofluorescence staining, fluorescence-activated cell sorting analysis, and biochemical measurements in the umbilical cord vessels and fetal red blood cells. Based on our data set, it is clear that the lower-weight siblings are markedly exposed to persistent intrauterine hypoxic conditions, which are connected to a decreased level in NOS3 activation. Furthermore, the increased level of peroxynitrite aggravates lipid peroxidation and induces morphological and functional damage and loss in redox homeostasis.
双胎妊娠率的上升给与妊娠并发症、宫内生长受限、围产期死亡率及合并症相关的临床实践带来了新的挑战。随着双胎妊娠的进展，对氧气供应的需求增加，这很容易破坏氧化还原稳态平衡，进而给发育中的胎儿带来更大挑战。显著的出生体重差异表明其中一个胎儿存在氧合或血流不足，这可能与生物可利用的一氧化氮水平较低有关。因此，在本研究中，我们聚焦于参与氧气供应调节的网络，如在出生体重差异较大的成熟双胎中诱导型一氧化氮合酶和内皮型一氧化氮合酶（NOS3）的激活以及自由基和脂质过氧化物的形成。通过免疫荧光染色、荧光激活细胞分选分析以及对脐带血管和胎儿红细胞进行生化测量来跟踪选定的参数。基于我们的数据集，很明显体重较轻的胎儿显著暴露于持续的宫内缺氧环境中，这与NOS3激活水平降低有关。此外，过氧亚硝酸盐水平的升高加剧了脂质过氧化，并导致氧化还原稳态的形态和功能损伤及丧失。
