Wang Zhiying, Cai Xiaojing, Ren Zhiyuan, Shao Yi, Xu Yongkang, Fu Lian, Zhu Yan
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, China.
Animals (Basel). 2023 Jul 21;13(14):2376. doi: 10.3390/ani13142376.
Pseudorabies virus (PRV) belongs to the family Herpesviridae. PRV has a wide host range and can cause cytopathic effects (CPEs) in PK-15 cells. Therefore, PRV was used as a model to study the antiviral activity of piceatannol. The results showed that piceatannol could restrain PRV multiplication in PK-15 cells in a dose-dependent manner. The 50% inhibitory concentration (IC) was 0.0307 mg/mL, and the selectivity index (SI, CC/IC) was 3.68. Piceatannol could exert an anti-PRV effect by reducing the transcription level of viral genes, inhibiting PRV-induced apoptosis and elevating the levels of IL-4, TNF-α and IFN-γ in the serum of mice. Animal experiments showed that piceatannol could delay the onset of disease, reduce the viral load in the brain and kidney and reduce the pathological changes in the tissues and organs of the mice to improve the survival rate of the mice (14.3%). Therefore, the anti-PRV activity of piceatannol in vivo and in vitro was systematically evaluated in this study to provide scientific data for developing a new alternative measure for controlling PRV infection.
伪狂犬病病毒（PRV）属于疱疹病毒科。PRV宿主范围广泛，可在PK - 15细胞中引起细胞病变效应（CPEs）。因此，PRV被用作研究白皮杉醇抗病毒活性的模型。结果表明，白皮杉醇能以剂量依赖性方式抑制PRV在PK - 15细胞中的增殖。50%抑制浓度（IC）为0.0307 mg/mL，选择性指数（SI，CC/IC）为3.68。白皮杉醇可通过降低病毒基因转录水平、抑制PRV诱导的细胞凋亡以及提高小鼠血清中IL - 4、TNF -α和IFN -γ水平发挥抗PRV作用。动物实验表明，白皮杉醇可延缓疾病发作，降低脑和肾中的病毒载量，减轻小鼠组织器官的病理变化，提高小鼠存活率（14.3%）。因此，本研究系统评价了白皮杉醇在体内和体外的抗PRV活性，为开发控制PRV感染的新替代措施提供科学数据。
