Ng Heng Kang, Puah Suat Moi, Teh Cindy Shuan Ju, Idris Nuryana, Chua Kek Heng
Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, Kuala Lumpur 50603, Malaysia.
Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur 50603, Malaysia.
Antibiotics (Basel). 2023 Jul 13;12(7):1185. doi: 10.3390/antibiotics12071185.
forms air-liquid interface pellicles that boost its ability to withstand desiccation and increase survival under antibiotic pressure. This study aims to delve into the transcriptomic profiles of pellicle cells from clinical strains of carbapenem-resistant (CRAB). The total RNA was extracted from pellicle cells from three pellicle-forming CRAB strains and planktonic cells from three non-pellicle-forming CRAB strains, subject to RNA sequencing using Illumina HiSeq 2500 system. A transcriptomic analysis between pellicle and planktonic cells, along with differential expression genes (DEGs) analysis and enrichment analysis of annotated COGs, GOs, and KEGGs, was performed. Our analysis identified 366 DEGs in pellicle cells: 162 upregulated genes and 204 downregulated genes. The upregulated ABUW_1624 () gene and downregulated ABUW_1550 gene indicated potential involvement in fatty acid degradation during pellicle formation. Another upregulated ABUW_2820 () gene, encoding the D-methionine transporter system, hinted at its contribution to pellicle formation. The upregulation of two-component systems, CusSR and KdpDE, which implies the regulation of copper and potassium ions in a CRAB pellicle formation was also observed. These findings provide valuable insights into the regulation of gene expression during the formation of pellicles in CRAB, and these are potential targets that may aid in the eradication of CRAB infections.
形成气液界面菌膜，增强其耐受干燥的能力，并提高在抗生素压力下的存活率。本研究旨在深入探究耐碳青霉烯类鲍曼不动杆菌（CRAB）临床菌株菌膜细胞的转录组谱。从三株形成菌膜的CRAB菌株的菌膜细胞和三株不形成菌膜的CRAB菌株的浮游细胞中提取总RNA，使用Illumina HiSeq 2500系统进行RNA测序。对菌膜细胞和浮游细胞进行转录组分析，同时进行差异表达基因（DEG）分析以及注释的COG、GO和KEGG的富集分析。我们的分析在菌膜细胞中鉴定出366个DEG：162个上调基因和204个下调基因。上调的ABUW_1624（）基因和下调的ABUW_1550基因表明在菌膜形成过程中可能参与脂肪酸降解。另一个上调的ABUW_2820（）基因编码D-甲硫氨酸转运系统，提示其对菌膜形成有贡献。还观察到双组分系统CusSR和KdpDE的上调，这意味着在CRAB菌膜形成中对铜离子和钾离子的调节。这些发现为CRAB菌膜形成过程中的基因表达调控提供了有价值的见解，并且这些是可能有助于根除CRAB感染的潜在靶点。
