Koreň Ján, Andrezál Michal, Ozaee Elham, Drahovská Hana, Wawruch Martin, Liptáková Adriána, Maliar Tibor
Institute of Microbiology, Faculty of Medicine, Comenius University, University Hospital Bratislava, 81108 Bratislava, Slovakia.
Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, 84215 Bratislava, Slovakia.
Antibiotics (Basel). 2023 Jul 20;12(7):1209. doi: 10.3390/antibiotics12071209.
The expansion of sequence type 131 (ST131) extended-spectrum β-lactamase (ESBL)-producing () represents major worldwide challenges. strains originating from healthcare facilities (labeled No. 1 and No. 2) of the University Hospital Bratislava (UHB) were analyzed for ST131 emergence, including its (sub)lineages and clonal relatedness. Antimicrobial resistance was determined in most strains. Of a total of 354 strains, 263 (74.3%) belonged to ST131; of these, 177 (67.3%) were from No. 1. Generally, among 260 ST131 , clades A/B were confirmed in 20 (7.7%), while clade C was noted in 240 (92.3%) strains; within them, subclades were detected as follows: C0 (17; 7.1%), C1 (3; 1.2%), and C2 (220; 91.7%). Among fifteen randomly selected strains that were investigated for ST and clonal relatedness, seven STs were identified: eight (53.3%) ST131, two (13.3%) ST73, and one each (6.7%) of ST10, ST12, ST14, ST1193, and ST1196. From No. 1, two ST131 in the first internal clinic and one ST131 from No. 2 in the aftercare department were highly clonally related, suggesting possible epidemiological association. Antimicrobial resistance was as follows: ciprofloxacin 93.8%, ceftazidime 78.4%, meropenem 0%, fosfomycin 2.9% and nitrofurantoin 1.4%. Prevention of ESBL-producing dissemination, especially for ST131 clade C2, is inevitably necessary for reducing drug resistance and decreasing healthcare-associated infections.
产生超广谱β-内酰胺酶（ESBL）的序列型131（ST131）大肠杆菌的传播是全球面临的主要挑战。对源自布拉迪斯拉发大学医院（UHB）医疗机构（标记为1号和2号）的大肠杆菌菌株进行了分析，以研究ST131的出现情况，包括其（亚）谱系和克隆相关性。对大多数菌株进行了耐药性测定。在总共354株大肠杆菌中，263株（74.3%）属于ST131；其中，177株（67.3%）来自1号。一般来说，在260株ST131大肠杆菌中，A/B分支在20株（7.7%）中得到确认，而C分支在240株（92.3%）菌株中被发现；在这些菌株中，检测到的亚分支如下：C0（17株；7.1%）、C1（3株；1.2%）和C2（220株；91.7%）。在随机选择的15株用于研究ST和克隆相关性的大肠杆菌菌株中，鉴定出7种序列型：8株（53.3%）为ST131，2株（13.3%）为ST73，以及ST10、ST12、ST14、ST1193和ST1196各1株（6.7%）。在1号的第一内科有2株ST131大肠杆菌，在2号的后续护理科有1株ST131大肠杆菌具有高度克隆相关性，提示可能存在流行病学关联。耐药情况如下：环丙沙星93.8%、头孢他啶78.4%、美罗培南0%、磷霉素2.9%和呋喃妥因1.4%。预防产ESBL大肠杆菌的传播，尤其是针对ST131 C2分支，对于降低耐药性和减少医疗相关感染是必不可少的。
