Mhaya Amel, Trabelsi Rahma, Aillerie Sabine, M'Zali Fatima, Bégu Dominique, Tounsi Slim, Gdoura Radhouane, Arpin Corinne
University of Bordeaux, Department of Biological and Medical Sciences, UMR CNRS 5234, 146 Rue Léo Saignat, 33076 Bordeaux, France.
Centre of Biotechnology of Sfax, Laboratory of Biopesticides, Road of Sidi Mansour Km 6, 3018 Sfax, Tunisia.
Antibiotics (Basel). 2022 Sep 29;11(10):1329. doi: 10.3390/antibiotics11101329.
During a two-month period (2017-2018), 336 urine samples positive for were collected from Tunisian patients. Of the 336 samples, 266 were collected from community patients and 70 from hospital settings. In all, 15 ESBL producers were identified (8 and 7, respectively) and assigned to 13 pulsotypes, including four ESBL-producing (ESBL-E) with E1 and E2 profiles (2 isolates each) from community patients. The two strains E1 were identified as B2-ST131 subclade C2 and the two isolates E2, A-ST617. The four strains carrying both CTX-M-15 and CTX-M-27, exhibited the multireplicon IncFII/F1A/F1B with the same formula F31:A4:B1. Two isolates with patterns E3 and E4 (Dice coefficient, 78.7%) isolated from community and hospital settings of two geographic areas were assigned to the emerging ST131 C1-M27 subclade and contained the replicon F1:A-:B20. The remaining ESBL-E divided into different sequence types/associated CTX-M: 2 ST131-C2/CTX-M-15 and ST744/CTX-M-55, ST617/CTM-15, ST2973/CTX-M-55, ST6448/CTX-M-15, ST224/CTX-M-15, ST1431/CTX-M-15, and ST38/CTX-M-27, one isolate each. Our study reports for the first time the presence in the Tunisian community of two clones of , including the virulent clone ST131-C2 harboring both CTX-M-15 and CTX-M-27, and confirms the spread of the emergent clone ST131-C1-M-27, notably in community urinary tract infections.
在2017年至2018年的两个月期间，从突尼斯患者中收集了336份对[具体物质]呈阳性的尿液样本。在这336份样本中，266份来自社区患者，70份来自医院环境。总共鉴定出15株产超广谱β-内酰胺酶（ESBL）菌株（分别为8株和7株），并分为13种脉冲型，其中包括4株来自社区患者的具有E1和E2型谱的产ESBL大肠杆菌（ESBL-E）（各2株）。两株E1菌株被鉴定为B2-ST131亚分支C2，两株E2分离株为A-ST617。携带CTX-M-15和CTX-M-27的四株菌株，呈现出具有相同公式F31:A4:B1的多复制子IncFII/F1A/F1B。从两个地理区域的社区和医院环境中分离出的两株具有E3和E4型谱（迪氏系数为78.7%）的分离株，被归为新兴的ST131 C1-M27亚分支，并含有复制子F1:A-:B20。其余的ESBL-E分为不同的序列类型/相关CTX-M：2株ST131-C2/CTX-M-15和ST744/CTX-M-55、ST617/CTM-15、ST2973/CTX-M-55、ST6448/CTX-M-15、ST224/CTX-M-15、ST1431/CTX-M-15和ST38/CTX-M-27，各1株。我们的研究首次报道了突尼斯社区中存在两种大肠杆菌克隆，包括携带CTX-M-15和CTX-M-27的毒力克隆ST131-C2，并证实了新兴克隆ST131-C1-M-27的传播，特别是在社区尿路感染中。
