Sex-Linked Growth Disorder and Aberrant Pituitary Gene Expression in Nestin-Cre-Mediated Conditional Knockout Mice.

Genes that regulate hormone release are essential for maintaining metabolism and energy balance. encodes a transcription factor that regulates hormone production and release, and a decreased in growth hormones has been reported in knockout mice. A reduction in growth hormones has also been observed in Nestin-Cre mice, a model frequently used to study the nervous system. Currently, it is unknown how loss or the Nestin-Cre driver disrupt pituitary gene expression. Here, we compared the growth curves and pituitary gene expression profiles of Nestin-Cre-mediated conditional knockout (cKO) mice with those of their controls. Reduced body weight was observed in both the Nestin-Cre and cKO mice, and the loss of had a slightly more severe impact on female mice than on male mice. RNA-seq data analyses revealed that the sex-related differences were amplified in the Nestin-Cre-mediated conditional knockout mice. Additionally, in the male mice, the influence of cKO on pituitary gene expression may be overridden by the Nestin-Cre driver. Differentially expressed genes associated with the Nestin-Cre driver were significantly enriched for genes related to growth factor activity and binding. Altogether, our results demonstrate that Nestin-Cre and the loss of in the neuronal cell lineage have distinct impacts on pituitary gene expression in a sex-specific manner.