Fei Jiao, Wang Youshao, Cheng Hao, Wang Hui, Wu Meilin, Sun Fulin, Sun Cuici
State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou 511458, China.
Bioengineering (Basel). 2023 Jul 24;10(7):878. doi: 10.3390/bioengineering10070878.
Aquaporins (AQPs) are essential channel proteins that play central roles in maintaining water homeostasis. Here, a novel aquaporin gene, named , was cloned from the mangrove plant by RACE technology. The gene was 1404 bp in length with an open reading frame (ORF) of 852 bp, encoded with 283 amino acids. Database comparisons revealed that protein shared the highest identity (91.26%) with the aquaporin , which was isolated from Gene expression analysis revealed that the gene was induced higher in leaves than in stems and roots of under cold stress. Transient expression of in epidermal cells revealed that the protein was localized to the plasma membrane. Overexpressing in significantly enhanced the lateral root number of the transgenic lines. transgenic demonstrated better growth, elevated proline content, increased superoxide dismutase (SOD) and peroxidase (POD) activities, and reduced malondialdehyde (MDA) content compared with the wild-type when exposed to cold stress. The findings suggest that overexpression of probably conferred cold tolerance of transgenic by enhancing osmoregulation and antioxidant capacity. This present data presents a valuable gene resource that contributes to the advancement of our understanding of aquaporins and their potential application in enhancing plant stress tolerance.
水通道蛋白（AQPs）是维持水平衡的关键通道蛋白。在此，通过RACE技术从红树林植物中克隆出一个名为的新型水通道蛋白基因。该基因长度为1404 bp，开放阅读框（ORF）为852 bp，编码283个氨基酸。数据库比较显示，该蛋白与从分离出的水通道蛋白具有最高的同一性（91.26%）。基因表达分析表明，在冷胁迫下，该基因在叶片中的诱导表达高于茎和根。在表皮细胞中的瞬时表达表明，该蛋白定位于质膜。在中过表达显著增加了转基因株系的侧根数。与野生型相比，转基因在冷胁迫下表现出更好的生长、脯氨酸含量升高、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性增加以及丙二醛（MDA）含量降低。研究结果表明，过表达可能通过增强渗透调节和抗氧化能力赋予转基因抗冷性。这些数据提供了宝贵的基因资源，有助于增进我们对水通道蛋白的理解及其在增强植物抗逆性方面的潜在应用。
