Lei Xiaojin, Tan Bing, Liu Zhongyuan, Wu Jing, Lv Jiaxin, Gao Caiqiu
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Front Plant Sci. 2021 May 17;12:653791. doi: 10.3389/fpls.2021.653791. eCollection 2021.
The CONSTANS-LIKE (COL) transcription factor has been reported to play important roles in regulating plant flowering and the response to abiotic stress. To clone and screen genes with excellent salt tolerance from the woody halophyte , 8 genes were identified in this study. The expression patterns of these genes under different abiotic stresses (high salt, osmotic, and heavy metal) and abscisic acid (ABA) treatment were detected using quantitative real-time PCR (qRT-PCR). The expression levels of 8 genes changed significantly after exposure to one or more stresses, indicating that these genes were all stress-responsive genes and may be involved in the stress resistance response of . In particular, the expression level of changed significantly at most time points in the roots and leaves of under salt stress and after ABA treatments, which may play an important role in the response process of salt stress through a mechanism dependent on the ABA pathway. The recombinant vectors pROKII- and pFGC5941- were constructed for the transient transformation of , and the transient infection of with the pROKII empty vector was used as the control to further verify whether the gene was involved in the regulation of the salt tolerance response of . Overexpression of the gene in plants under 150 mM NaCl stress increased the ability of transgenic cells to remove reactive oxygen species (ROS) by regulating the activity of protective enzymes and promoting a decrease in the accumulation of O and HO, thereby reducing cell damage or cell death and enhancing salt tolerance. The gene may be a candidate gene associated with excellent salt tolerance. Furthermore, the expression levels of some genes related to the ABA pathway were analyzed using qRT-PCR. The results showed that the expressions of and were significantly higher, and the expressions of , , and 3 were not significantly altered in OE compared with CON under normal conditions. But after 24 h of salt stress, the expressions of all five studied genes all were lower than the normal condition. In the future, the downstream genes directly regulated by the transcription factor will be searched and identified to analyze the salt tolerance regulatory network of .
据报道，CONSTANS类（COL）转录因子在调节植物开花和对非生物胁迫的响应中发挥重要作用。为了从木本盐生植物中克隆和筛选具有优异耐盐性的基因，本研究鉴定了8个基因。使用定量实时PCR（qRT-PCR）检测这些基因在不同非生物胁迫（高盐、渗透和重金属）和脱落酸（ABA）处理下的表达模式。暴露于一种或多种胁迫后，8个基因的表达水平发生了显著变化，表明这些基因都是胁迫响应基因，可能参与了[植物名称]的抗逆反应。特别是，[基因名称]在[植物名称]的根和叶中，在盐胁迫和ABA处理后的大多数时间点表达水平显著变化，这可能通过依赖ABA途径的机制在盐胁迫响应过程中发挥重要作用。构建了重组载体pROKII-[基因名称]和pFGC5941-[基因名称]用于[植物名称]的瞬时转化，并以pROKII空载体对[植物名称]的瞬时感染作为对照，进一步验证[基因名称]是否参与调节[植物名称]的耐盐性反应。在150 mM NaCl胁迫下，植物中[基因名称]的过表达通过调节保护酶的活性和促进O和HO积累的减少，提高了转基因[植物名称]细胞清除活性氧（ROS）的能力，从而减少细胞损伤或细胞死亡并增强耐盐性。[基因名称]可能是与优异耐盐性相关的候选基因。此外，使用qRT-PCR分析了一些与ABA途径相关基因的表达水平。结果表明，在正常条件下，与对照相比，[基因名称1]和[基因名称2]的表达显著更高，[基因名称3]、[基因名称4]和[基因名称5]的表达在过表达植株中没有显著变化。但在盐胁迫24小时后，所有五个研究基因的表达均低于正常条件。未来，将搜索和鉴定由[转录因子名称]直接调控的下游基因，以分析[植物名称]的耐盐调控网络。
