Loinjak Domagoj, Mihić Damir, Smolić Robert, Maričić Lana, Šahinović Ines, Smolić Martina, Sikora Renata, Loinjak Sanja, Dinjar Kristijan, Včev Aleksandar
Faculty of Medicine, University J. J. Strossmayer in Osijek, 31000 Osijek, Croatia.
Department of Pulmology and Intensive Care Medicine, University Hospital Centre Osijek, 31000 Osijek, Croatia.
Biomedicines. 2023 Jun 27;11(7):1847. doi: 10.3390/biomedicines11071847.
Acute respiratory distress syndrome (ARDS) is one of the most severe complications of the COVID-19 disease. The role of IL-33 and calpain 1 was previously described in lung infections and lung tissue damage. Our study examined the association between serum calpain 1 activity and IL-33 concentration in patients with COVID-19 ARDS. In the research, we included 80 subjects who had COVID-19 pneumonia and divided them into 2 groups: 40 subjects with ARDS and 40 subjects without ARDS. The basis of the research was the collection of subjects' data and the sampling of peripheral venous blood. The concentration of IL-33 was determined by the ELISA method and the activity of calpain 1 by the fluorometry method. Our research showed elevated calpain 1 activity and IL-33 concentration in the serum of COVID-19 patients who developed ARDS compared to those who did not develop ARDS and a positive correlation between them was established. Further, a positive correlation was established between the examined parameters and the severity of the disease, proinflammatory markers, and the use of mechanical ventilation. These results indicate a possible association and role of calpain 1 and IL-33 with the development of ARDS in COVID-19 patients.
急性呼吸窘迫综合征（ARDS）是新型冠状病毒肺炎（COVID-19）最严重的并发症之一。白细胞介素-33（IL-33）和钙蛋白酶1在肺部感染和肺组织损伤中的作用此前已有描述。我们的研究检测了COVID-19相关ARDS患者血清钙蛋白酶1活性与IL-33浓度之间的关联。在这项研究中，我们纳入了80例COVID-19肺炎患者，并将他们分为两组：40例ARDS患者和40例非ARDS患者。本研究的基础是收集受试者数据并采集外周静脉血样本。采用酶联免疫吸附测定（ELISA）法测定IL-33浓度，采用荧光测定法测定钙蛋白酶1活性。我们的研究表明，与未发生ARDS的COVID-19患者相比，发生ARDS的COVID-19患者血清中钙蛋白酶1活性和IL-33浓度升高，且二者之间存在正相关。此外，所检测的参数与疾病严重程度、促炎标志物以及机械通气的使用之间也存在正相关。这些结果表明钙蛋白酶1和IL-33与COVID-19患者ARDS的发生可能存在关联并发挥作用。
