Shivanna Komala, Astumian Mary, Raut Prakash, Ngo Vinh-Nhan, Hess Samuel T, Henry Clarissa
Department of Physics & Astronomy, University of Maine, 5709 Bennett Hall, Orono, ME 04469-5709, USA.
School of Biology and Ecology, University of Maine, 217 Hitchner Hall, Orono, ME 04469-5751, USA.
Biomedicines. 2023 Jul 8;11(7):1941. doi: 10.3390/biomedicines11071941.
Cell signaling is determined partially by the localization and abundance of proteins. Dystroglycan and integrin are both transmembrane receptors that connect the cytoskeleton inside muscle cells to the extracellular matrix outside muscle cells, maintaining proper adhesion and function of muscle. The position and abundance of Dystroglycan relative to integrins is thought to be important for muscle adhesion and function. The subcellular localization and quantification of these receptor proteins can be determined at the nanometer scale by FPALM super-resolution microscopy. We used FPALM to determine localizations of Dystroglycan and integrin proteins in muscle fibers of intact zebrafish (). Results were consistent with confocal imaging data, but illuminate further details at the nanoscale and show the feasibility of using FPALM to quantify interactions of two proteins in a whole organism.
细胞信号传导部分由蛋白质的定位和丰度决定。肌营养不良蛋白聚糖和整合素都是跨膜受体，它们将肌肉细胞内部的细胞骨架与肌肉细胞外部的细胞外基质相连，维持肌肉的正常粘附和功能。肌营养不良蛋白聚糖相对于整合素的位置和丰度被认为对肌肉粘附和功能很重要。这些受体蛋白的亚细胞定位和定量可以通过FPALM超分辨率显微镜在纳米尺度上确定。我们使用FPALM来确定完整斑马鱼肌肉纤维中肌营养不良蛋白聚糖和整合素蛋白的定位。结果与共聚焦成像数据一致，但在纳米尺度上揭示了更多细节，并显示了使用FPALM在整个生物体中量化两种蛋白质相互作用的可行性。
