Seo Min Hyung, Yeo Sujung
Department of Meridian and Acupoint, College of Korean Medicine, Sang Ji University, Wonju 26339, Republic of Korea.
Research Institute of Korean Medicine, Sang Ji University, #83 Sangjidae-Gil, Wonju 26339, Republic of Korea.
Biomedicines. 2023 Jul 10;11(7):1952. doi: 10.3390/biomedicines11071952.
Our understanding of the gastrointestinal system in the pathophysiology of Parkinson's disease (PD) has grown considerably over the last two decades. Patients with PD experience notable gastrointestinal symptoms, including constipation. In this study, the effects of knocked-down serping1, associated with the contraction and relaxation of smooth muscle and inflammation responses, by applying the serping1 siRNA were investigated in 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine-induced PD mice in an α-syn change aspect. In the result, serping1 expression was knocked down by the treatment of serping1 siRNA, and decreased serping1 induced the decrease α-syn in the colon. Furthermore, the changes in α-syn aggregation were also examined in the brain, and alleviated α-syn aggregation was also observed in an siRNA treatment group. The results indicated that serping1 siRNA could ease synucleinopathy related to the gastrointestinal system in PD. This study also raises the possibility that serping1 siRNA could alleviate α-syn aggregation in striatum and substantia nigra regions of the brain.
在过去二十年中，我们对帕金森病（PD）病理生理学中胃肠道系统的理解有了显著增长。PD患者会出现明显的胃肠道症状，包括便秘。在本研究中，通过应用serping1小干扰RNA（siRNA），在1-甲基-4-苯基-1,2,3,6-四氢吡啶诱导的PD小鼠中，从α-突触核蛋白变化方面研究了与平滑肌收缩和舒张以及炎症反应相关的serping1基因敲低的影响。结果显示，serping1 siRNA处理可使serping1表达降低，serping1表达降低导致结肠中α-突触核蛋白减少。此外，还检测了大脑中α-突触核蛋白聚集的变化，在siRNA处理组中也观察到α-突触核蛋白聚集减轻。结果表明，serping1 siRNA可缓解PD中与胃肠道系统相关的突触核蛋白病。本研究还提出了serping1 siRNA可减轻大脑纹状体和黑质区域α-突触核蛋白聚集的可能性。
