Blyth W, Stewart G E
Mycopathologia. 1978 Dec 29;66(1-2):51-7. doi: 10.1007/BF00429593.
The principal ultrastructural changes in cells of Candida albicans treated with amphotericin B (AmB), either in vitro or in vivo, and in the presence or absence of prednisolone included plasmolysis, vacuolation and destruction of organelles. Lamination of the cell wall, although discernible after 4 h antibiotic treatment in vitro, was conspicuous in vivo, especially in prednisolone-treated mice given AmB 72 h after inoculation and was seen in both phagocytosed cells and those free in inflammatory exudates. Somatic extracts from control cells and somatic extracts and leachates from AmB-treated cells showed the presence of low-grade toxic components when given i.p. to mice receiving antinomycin D(AMD) s.c. Culture filtrates were negative. Eighteen hour cultures were more toxic than those grown for 3 days and no toxicity was shown for cultures after 8 or 14 days. The behaviour of toxic materials during electrophoresis in polyacrylamide gels suggested that they were proteins of relatively high molecular weight.
在体外或体内用两性霉素B（AmB）处理白色念珠菌细胞时，无论是否存在泼尼松龙，主要的超微结构变化包括质壁分离、空泡化和细胞器破坏。细胞壁的分层，虽然在体外抗生素处理4小时后可辨别，但在体内很明显，尤其是在接种后72小时给予AmB的泼尼松龙处理的小鼠中，并且在吞噬细胞和炎症渗出物中游离的细胞中都可见。对照细胞的体细胞提取物以及AmB处理细胞的体细胞提取物和渗滤液，当腹腔注射给皮下注射放线菌素D（AMD）的小鼠时，显示存在低毒成分。培养滤液为阴性。18小时的培养物比培养3天的培养物毒性更大，8天或14天后的培养物没有显示出毒性。有毒物质在聚丙烯酰胺凝胶电泳过程中的行为表明它们是相对高分子量的蛋白质。
