Diamantidou Dimitra, Tsochatzis Emmanouil, Kalogiannis Stavros, Alberto Lopes Joao, Theodoridis Georgios, Gika Helen
Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Biomic_AUTh, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, B1.4, Thessaloniki, 10th km Thessaloniki-Thermi Rd, P.O. Box 8318, 57001 Thermi, Greece.
Foods. 2023 Jul 19;12(14):2739. doi: 10.3390/foods12142739.
Oligomers are a particular category of non-intentionally added substances (NIAS) that may be present in food contact materials (FCMs), such as polyethylene terephthalate (PET), and consequently migrate into foods. Here, an ultra-high-pressure liquid chromatography quadruple time-of-flight mass spectrometry (UHPLC-qTOF-MS) method was developed for the analysis of 1st series cyclic PET oligomers in virgin olive oil (VOO) following a QuEChERS clean-up protocol. Oligomer migration was evaluated with two different migration experiments using bottles from virgin and recycled PET: one with VOO samples stored in household conditions for a year and one using the food simulant D2 (95% / ethanol in water) at 60 °C for 10 days. Calibration curves were constructed with fortified VOO samples, with the LOQs ranging from 10 to 50 µg L and the recoveries ranging from 86.6 to 113.0%. Results showed no migration of PET oligomers in VOO. However, in the simulated study, significant amounts of all oligomers were detected, with the migration of cyclic PET trimers from recycled bottles being the most abundant. Additional substances were tentatively identified as linear derivatives of PET oligomers. Again, open trimer structures in recycled bottles gave the most significant signals.
低聚物是一类特殊的非有意添加物质（NIAS），可能存在于食品接触材料（FCM）中，如聚对苯二甲酸乙二酯（PET），并因此迁移到食品中。在此，开发了一种超高压液相色谱四极杆飞行时间质谱（UHPLC-qTOF-MS）方法，用于按照QuEChERS净化方案分析初榨橄榄油（VOO）中的第一系列环状PET低聚物。使用来自原生和回收PET的瓶子进行了两个不同的迁移实验，以评估低聚物的迁移情况：一个实验是将VOO样品在家庭条件下储存一年，另一个实验是在60°C下使用食品模拟物D2（水中95%乙醇）处理10天。用加标VOO样品构建校准曲线，定量限范围为10至50 μg/L，回收率范围为86.6%至113.0%。结果表明，VOO中未检测到PET低聚物的迁移。然而，在模拟研究中，检测到了大量的所有低聚物，其中回收瓶中环状PET三聚体的迁移量最大。还初步鉴定出其他物质为PET低聚物的线性衍生物。同样，回收瓶中的开放三聚体结构给出了最显著的信号。
