Jiang Guihun, Ramachandraiah Karna, Tan Chaoyi, Cai Nanjie, Ameer Kashif, Feng Xiaoyu
School of Public Health, Jilin Medical University, Jilin 132013, China.
School of Lifesciences, Sejong University, Seoul 05006, Republic of Korea.
Foods. 2023 Jul 24;12(14):2809. doi: 10.3390/foods12142809.
In this study, the effects of enzymatic modification using cellulase/xylanase on the composition and structural and functional properties of ginseng insoluble dietary fiber (G-IDF) were evaluated. Fourier transform infrared spectroscopy and scanning electron microcopy showed that enzymatic extraction treatment caused obvious structural alterations in ginseng-modified (G-MIDF) samples, which exhibited more porous and completely wrinkled surfaces. Comparing the peak morphology of G-MIDF with untreated IDF using X-ray diffractometry, the G-MIDF sample exhibited split peaks at a 2θ angle of 23.71°, along with the emergence of sharp peaks at 28.02°, 31.78°, and 35.07°. Thermo-gravimetric analysis showed that G-MIDF exhibited a specified range of pyrolysis temperature and is suitable for food applications involving processing at temperatures below 300 °C. Overall, it was evident from rheograms that both G-IDF and G-MIDF exhibited a resemblance with respect to viscosity changes as a function of the shear rate. Enzymatic treatment led to significant ( < 0.05) improvement in water holding, oil retention, water swelling, nitrite ion binding, bile acid binding, cholesterol absorption, and glucose absorption capacities.
在本研究中,评估了使用纤维素酶/木聚糖酶进行酶促修饰对人参不溶性膳食纤维(G-IDF)的组成、结构和功能特性的影响。傅里叶变换红外光谱和扫描电子显微镜表明,酶促提取处理导致人参改性(G-MIDF)样品出现明显的结构变化,其表面呈现出更多的孔隙和完全褶皱。使用X射线衍射仪比较G-MIDF与未处理的IDF的峰形态,G-MIDF样品在2θ角为23.71°处出现分裂峰,同时在28.02°、31.78°和35.07°处出现尖锐峰。热重分析表明,G-MIDF具有特定的热解温度范围,适用于温度低于300℃的食品加工应用。总体而言,流变图显示,G-IDF和G-MIDF在粘度随剪切速率变化方面表现出相似性。酶处理导致持水、持油、水溶胀、亚硝酸根离子结合、胆汁酸结合、胆固醇吸收和葡萄糖吸收能力显著(<0.05)提高。