Applied Biotechnology Center, Wuhan University of Bioengineering, Wuhan 430415, China.
College of Life Science and Technology, Wuhan University of Bioengineering, Wuhan 430415, China.
Genes (Basel). 2023 Jun 27;14(7):1361. doi: 10.3390/genes14071361.
Seed germination is the complex adaptive trait of higher plants influenced by a large number of genes and environmental factors. Numerous studies have been performed to better understand how germination is controlled by various environmental factors and applied chemicals, such as cyanide. However, still very little is known about the molecular mechanisms of how extrinsic signals regulate seed germination. Our and previous studies found that non-lethal cyanide treatment promotes seed germination, but the regulatory mechanism is unclear. In this study, we found that a low concentration of cyanide pretreatment significantly enhanced the expression of endo-β-mannanase 5 () gene in , and the mutation of this gene impaired cyanide-mediated seed germination. In contrast, overexpression of gene enhanced Arabidopsis seed germination ability under both normal and salt stress conditions. Further studies showed that the expression of the gene was negatively regulated by ABA insensitive 5 (ABI5); In mutant seeds, the expression of the gene was increased and the seed germination rate was accelerated. Additionally, cyanide pretreatment markedly reduced the gene expression of in Arabidopsis seeds. Taken together, our data support the involvement of as a key gene in cyanide-mediated seed germination and confirm the role of ABI5 as a critical negative factor involved in cyanide-regulated gene expression.
种子萌发是高等植物的复杂适应性特征，受大量基因和环境因素的影响。已经进行了许多研究来更好地理解萌发如何受到各种环境因素和应用化学物质（如氰化物）的控制。然而，对于外部信号如何调节种子萌发的分子机制仍然知之甚少。我们和以前的研究发现，低浓度的氰化物预处理显著促进了 中内-β-甘露聚糖酶 5()基因的表达，而该基因的突变损害了氰化物介导的种子萌发。相比之下，过表达 基因增强了拟南芥在正常和盐胁迫条件下的种子萌发能力。进一步的研究表明，基因的表达受非生物胁迫 5（ABI5）的负调控；在 突变体种子中，基因的表达增加，种子萌发率加快。此外，氰化物预处理显著降低了拟南芥种子中基因的表达。综上所述，我们的数据支持 作为氰化物介导种子萌发的关键基因之一，并证实 ABI5 作为参与氰化物调节基因表达的关键负因子的作用。
