National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Genes (Basel). 2023 Jul 16;14(7):1454. doi: 10.3390/genes14071454.
is an exoribonuclease that degrades mRNA in the cytoplasm along the 5'-3' direction. A previous study indicated that it may be involved in the reproduction of . Quantitative real-time PCR was used to detect the spatiotemporal expression pattern of . At the tissue level, was significantly expressed in the ovary. During development, was significantly expressed at the CS stage of the embryo, on the 10th day post-larval and in the O2 stage of ovarian reproduction. The in situ hybridization results showed the location of in the ovary. The expression of was significantly increased after in vivo injection of dsRNA. This suggests that negatively regulates the expression of . Furthermore, we counted the number of at various stages of ovarian reproduction on different days after RNAi. The results showed that ovarian development was significantly accelerated. In general, the results of the present study indicate that has an inhibitory effect on the ovarian maturation of . The inhibitory effect might be through negative regulation of .
是一种在细胞质中沿着 5'-3'方向降解 mRNA 的外核核酸酶。先前的研究表明,它可能参与 的复制。定量实时 PCR 用于检测 的时空表达模式。在组织水平上, 在卵巢中显著表达。在发育过程中, 在胚胎的 CS 期、幼虫后第 10 天和卵巢生殖的 O2 期显著表达。原位杂交结果显示了 在卵巢中的位置。体内注射 dsRNA 后, 的表达显著增加。这表明 负调控 的表达。此外,我们在 RNAi 后不同天数的卵巢生殖的各个阶段计数 的数量。结果表明,卵巢发育明显加快。总的来说,本研究的结果表明, 在 的卵巢成熟中具有抑制作用。抑制作用可能是通过 负调控实现的。