Shandong Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian 271018, China.
Genes (Basel). 2023 Jul 19;14(7):1477. doi: 10.3390/genes14071477.
Quantitative real-time PCR (qRT-PCR) in sweet potatoes requires accurate data normalization; however, there are insufficient studies on appropriate reference genes for gene expression analysis. We examined variations in the expression of eight candidate reference genes in the leaf and root tissues of sweet potatoes (eight nonvirus-infected or eight virus-infected samples). Parallel analyses with geNorm, NormFinder, and Best-Keeper show that different viral infections and origin tissues affect the expression levels of these genes. Based on the results of the evaluation of the three software, the is suitable for nonvirus or virus-infected sweet potato leaves. and extension proteins are suitable for nonvirus-infected sweet potato leaves. is suitable for virus-infected sweet potato leaves. is suitable for roots of nonvirus-infected sweet potatoes. is suitable for virus-infected sweet potato roots. The research provides appropriate reference genes for further analysis in leaf and root samples of viruses in sweet potatoes.
甘薯中定量实时 PCR(qRT-PCR)需要准确的数据归一化；然而，用于基因表达分析的合适参考基因的研究还不够充分。我们研究了甘薯叶片和根组织中 8 个候选参考基因的表达变化(8 个非病毒感染或 8 个病毒感染样本)。使用 geNorm、NormFinder 和 Best-Keeper 进行平行分析表明，不同的病毒感染和来源组织会影响这些基因的表达水平。根据这三种软件的评价结果，在非病毒或病毒感染的甘薯叶片中， 是合适的。 和 延伸蛋白适合非病毒感染的甘薯叶片。 适合病毒感染的甘薯叶片。 适合非病毒感染的甘薯根。 适合病毒感染的甘薯根。该研究为进一步分析甘薯叶片和根组织中的病毒提供了合适的参考基因。
