Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China.
College of Animal Science, Guizhou University, Guiyang 550025, China.
Genes (Basel). 2023 Jul 22;14(7):1498. doi: 10.3390/genes14071498.
Myocyte enhancer factor 2A () is a member of the myocyte enhancer factor 2 family. is widely distributed in various tissues and organs and participates in various physiological processes. This study aimed to investigate the effect of expression on the proliferation and apoptosis of bovine myoblasts. CCK8, ELISA, cell cycle, and apoptosis analyses were conducted to assess cell status. In addition, the mRNA expression levels of genes associated with bovine myoblast proliferation and apoptosis were evaluated using RT-qPCR. The results showed that the upregulation of mRNA promoted the proliferation rate of myoblasts, shortened the cycle process, and increased the anti-apoptotic rate. Furthermore, the RT-qPCR results showed that the upregulation of mRNA significantly increased the cell proliferation factors and , cell cycle factors and , and the apoptotic factors and ( < 0.01). These results show that the gene can positively regulate myoblast proliferation and anti-apoptosis, providing a basis for the analysis of the regulatory mechanism of the gene on bovine growth and development.
肌细胞增强因子 2A () 是肌细胞增强因子 2 家族的一员。 在各种组织和器官中广泛分布，参与各种生理过程。本研究旨在探讨 表达对牛肌母细胞增殖和凋亡的影响。通过 CCK8、ELISA、细胞周期和凋亡分析来评估细胞状态。此外，还使用 RT-qPCR 评估了与牛肌母细胞增殖和凋亡相关的基因的 mRNA 表达水平。结果表明，上调 mRNA 促进了肌母细胞的增殖率，缩短了周期过程，并提高了抗凋亡率。此外，RT-qPCR 结果表明，上调 mRNA 显著增加了细胞增殖因子 和 、细胞周期因子 和 ，以及凋亡因子 和 ( < 0.01)。这些结果表明， 基因可以正向调节肌母细胞的增殖和抗凋亡，为分析 基因对牛生长发育的调控机制提供了依据。
