Effect of Bovine Gene Expression on Proliferation and Apoptosis of Myoblast Cells.

Myocyte enhancer factor 2A () is a member of the myocyte enhancer factor 2 family. is widely distributed in various tissues and organs and participates in various physiological processes. This study aimed to investigate the effect of expression on the proliferation and apoptosis of bovine myoblasts. CCK8, ELISA, cell cycle, and apoptosis analyses were conducted to assess cell status. In addition, the mRNA expression levels of genes associated with bovine myoblast proliferation and apoptosis were evaluated using RT-qPCR. The results showed that the upregulation of mRNA promoted the proliferation rate of myoblasts, shortened the cycle process, and increased the anti-apoptotic rate. Furthermore, the RT-qPCR results showed that the upregulation of mRNA significantly increased the cell proliferation factors and , cell cycle factors and , and the apoptotic factors and ( < 0.01). These results show that the gene can positively regulate myoblast proliferation and anti-apoptosis, providing a basis for the analysis of the regulatory mechanism of the gene on bovine growth and development.