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为少精子症、癌症及其他病症患者储存精子的男性精液冷冻保存：一家意大利精子库的24年经验

Semen Cryopreservation for Men Banking for Oligozoospermia, Cancers, and Other Conditions: 24 Years' Experience of an Italian Bank.

作者信息

Marchiani Sara, Degl'Innocenti Selene, Dabizzi Sara, Tamburrino Lara, Fino Maria Grazia, Traini Giulia, Calamai Costanza, Maggi Mario, Vignozzi Linda, Baldi Elisabetta, Lotti Francesco

机构信息

Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, 50134 Florence, Italy.

Andrology, Women's Endocrinology and Gender Incongruence Unit, Center for Prevention, Diagnosis and Treatment of Infertility, Careggi University Hospital, 50139 Florence, Italy.

出版信息

J Clin Med. 2023 Jul 13;12(14):4657. doi: 10.3390/jcm12144657.

DOI:10.3390/jcm12144657
PMID:37510772
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10380851/
Abstract

BACKGROUND

Sperm cryopreservation is recommended to preserve male fertility for cancer patients or other medical conditions at risk of sperm decline. Whether motility and viability recovery rates vary depending on the medical conditions requiring cryopreservation is poorly known. We report here on the 24-year experience of our semen bank.

METHODS

Motility and viability recovery rates were evaluated in 1973 collections from patients with various medical conditions and 67 collections from donors, and the results were related to basal semen quality.

RESULTS

Motility and viability recovery were highly related to basal semen quality and varied between cancer and non-cancer conditions, independently of the duration of cryopreservation and patient age. In samples with a sperm number below 2 × 10/mL, recovery rates approximated to zero. The highest recovery rates were found in donor collections. Cut-off values for the recovery of at least 1% motile spermatozoa were established based on initial semen quality.

CONCLUSIONS

Our results indicate that the occurrence of any pathological or medical condition resulted in lower recovery rates with respect to donors, indicating that intrinsic sperm characteristics drive susceptibility to cryodamage. Established cut-off values for motility recovery can be useful for patient counseling as well as for ART laboratories to decide the type of procedure.

摘要

背景

对于癌症患者或其他存在精子数量下降风险的疾病患者，建议进行精子冷冻保存以保留男性生育能力。目前对于因需要冷冻保存的疾病状况不同，精子活力和存活率的恢复率是否存在差异知之甚少。我们在此报告我们精液库24年的经验。

方法

对1973例患有各种疾病的患者的精液样本以及67例供体的精液样本的活力和存活率恢复率进行了评估，并将结果与基础精液质量相关联。

结果

活力和存活率的恢复与基础精液质量高度相关，并且在癌症和非癌症疾病状况之间存在差异，与冷冻保存时间和患者年龄无关。在精子数量低于2×10⁶/mL的样本中，恢复率接近零。供体精液样本的恢复率最高。根据初始精液质量确定了至少1%活动精子恢复率的临界值。

结论

我们的结果表明，任何病理或疾病状况的出现都会导致与供体相比恢复率降低，这表明精子的内在特征决定了对冷冻损伤的易感性。确定的活力恢复临界值可用于患者咨询以及辅助生殖技术实验室决定操作类型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/285a2f43df8f/jcm-12-04657-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/34a747f515c4/jcm-12-04657-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/af51634d744a/jcm-12-04657-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/64d77c6959d7/jcm-12-04657-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/2643e8e32f36/jcm-12-04657-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/0488d43fee13/jcm-12-04657-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/285a2f43df8f/jcm-12-04657-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/34a747f515c4/jcm-12-04657-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/af51634d744a/jcm-12-04657-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/64d77c6959d7/jcm-12-04657-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/2643e8e32f36/jcm-12-04657-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/0488d43fee13/jcm-12-04657-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b956/10380851/285a2f43df8f/jcm-12-04657-g006.jpg

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