Andrade Maria B R, Bertolla Ricardo P, Intasqui Paula, Antoniassi Mariana P, Tibaldi Danielle S, Belardin Larissa B, Spaine Deborah M
Division of Urology, Department of Surgery, Human Reproduction Section, Sao Paulo Federal University - Sao Paulo Hospital, Sao Paulo, Brazil.
Andrologia. 2019 Apr;51(3):e13205. doi: 10.1111/and.13205. Epub 2018 Nov 29.
To verify if quality of spermatozoa from men with testicular germ cell tumours is better before or after orchiectomy. This prospective study was carried out including 24 patients with testicular germ cell tumours, who provide one semen sample before they were submitted to unilateral orchiectomy and one other semen sample 30 days after the surgery. After collection by masturbation and liquefaction, an aliquot of the semen sample was used for semen analysis and another was used to evaluate sperm mitochondrial activity, DNA fragmentation and acrosome integrity. Seminal plasma was used to evaluate lipid peroxidation levels. Pre-orchiectomy sample and post-orchiectomy sample were compared using a paired Student's t test (normal distribution) or a paired Wilcoxon test, when appropriate (p ˂ 0.05). No significant difference was observed in semen analysis. A significant decrease in DNA fragmentation and lipid peroxidation and an increase in mitochondrial activity were observed after orchiectomy. Based on our findings, the semen quality from men with testicular germ cell tumours is better after orchiectomy.
为验证睾丸生殖细胞肿瘤患者在睾丸切除术前或术后精子质量是否更佳。本前瞻性研究纳入了24例睾丸生殖细胞肿瘤患者，他们在接受单侧睾丸切除术之前提供一份精液样本，并在术后30天提供另一份精液样本。通过手淫采集精液并液化后，取一份精液样本用于精液分析，另一份用于评估精子线粒体活性、DNA片段化和顶体完整性。精浆用于评估脂质过氧化水平。术前样本和术后样本在适当情况下使用配对t检验（正态分布）或配对Wilcoxon检验进行比较（p＜0.05）。精液分析未观察到显著差异。睾丸切除术后观察到DNA片段化和脂质过氧化显著降低，线粒体活性增加。基于我们的研究结果，睾丸生殖细胞肿瘤患者在睾丸切除术后精液质量更佳。
