College of Agronomy, Hunan Agricultural University, Changsha 410128, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Int J Mol Sci. 2023 Jul 14;24(14):11454. doi: 10.3390/ijms241411454.
Plant height is one of the most crucial components of plant structure. However, due to its complexity, the genetic architecture of rice plant height has not been fully elucidated. In this study, we performed a genome-wide association study (GWAS) to determine rice plant height using 178 commercial rice varieties and identified 37 loci associated with rice plant height (LAPH). Among these loci, in LAPH2, we identified a polygalacturonase gene, , which was genetically and functionally associated with rice plant height. The rice plant exhibits a super dwarf phenotype when the knockout of the gene occurs via CRISPR-Cas9 gene-editing technology. RNA-Seq analysis indicated that modulates the expression of genes involved in phytohormone metabolism and cell-wall-biosynthesis pathways. Our findings suggest that plays a vital role in controlling rice plant height by regulating cell wall biosynthesis. Given that rice architecture is one of the most critical phenotypes in rice breeding, has potential in rice's molecular design breeding toward an ideal plant height.
株高是植物结构的最重要组成部分之一。然而，由于其复杂性，水稻株高的遗传结构尚未完全阐明。在这项研究中，我们使用 178 个商业水稻品种进行了全基因组关联研究（GWAS），以确定水稻株高，并鉴定了与水稻株高相关的 37 个位点（LAPH）。在这些位点中，我们在 LAPH2 中鉴定了一个多聚半乳糖醛酸酶基因 ，它在遗传和功能上与水稻株高相关。当通过 CRISPR-Cas9 基因编辑技术敲除 基因时，水稻植株表现出超矮秆表型。RNA-Seq 分析表明， 调节参与植物激素代谢和细胞壁生物合成途径的基因的表达。我们的研究结果表明， 通过调节细胞壁生物合成在控制水稻株高方面起着至关重要的作用。鉴于水稻的结构是水稻育种中最关键的表型之一， 在理想株高的水稻分子设计育种中具有潜力。
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