Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, Japan.
Department of Patient Safety and Healthcare Administration, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, Japan.
Int J Mol Sci. 2023 Jul 16;24(14):11532. doi: 10.3390/ijms241411532.
While research suggests that increasing body mass index (BMI) is a risk factor for hip osteoarthritis (HOA), the mechanisms of this effect are not fully understood. Tryptases are among the main proteases found in mast cells (MCs) and contribute to OA pathology. , which encodes β-tryptase, is increased in the synovium of overweight and obese knee OA patients. However, it remains unclear whether tryptase in the synovium of HOA is increased with increasing BMI. Here, we investigated tryptase genes ( and ) in the synovium of overweight HOA patients. Forty-six patients radiographically diagnosed with HOA were allocated to two groups based on BMI, namely normal (<25 kg/m) and overweight (25-29.99 kg/m). and expression in the synovium of the two groups was compared using real-time polymerase chain reaction. To compare and expression in MCs between the groups, we isolated the MC-rich fraction (MC-RF) and MC-poor fraction (MC-PF), extracted using magnetic isolation. and expression was increased in the overweight group compared with the normal group. Expression of both genes in the MC-RF was significantly higher than that in MC-PF in both groups. However, and expression levels in the MC-RF did not differ between the groups. Tryptase genes were highly expressed in the synovium of overweight HOA patients. Further investigation to reveal the role of tryptase in the relationship between increasing BMI and HOA pathology is required.
虽然研究表明，体重指数（BMI）的增加是髋关节骨关节炎（HOA）的一个风险因素，但这种影响的机制尚不完全清楚。类胰蛋白酶是肥大细胞（MCs）中发现的主要蛋白酶之一，有助于 OA 病理。编码β-类胰蛋白酶的基因在超重和肥胖的膝骨关节炎患者的滑膜中增加。然而，尚不清楚 HOA 滑膜中的类胰蛋白酶是否随着 BMI 的增加而增加。在这里，我们研究了超重 HOA 患者滑膜中的类胰蛋白酶基因（和）。46 名经影像学诊断为 HOA 的患者根据 BMI 分为两组，即正常（<25 kg/m）和超重（25-29.99 kg/m）。使用实时聚合酶链反应比较两组滑膜中和的表达。为了比较两组 MC 之间和的表达，我们使用磁分离法分离富含 MC 的部分（MC-RF）和 MC 贫部分（MC-PF）。与正常组相比，超重组中表达增加。两组中，MC-RF 中两个基因的表达均显著高于 MC-PF。然而，两组 MC-RF 中的和表达水平没有差异。类胰蛋白酶基因在超重 HOA 患者的滑膜中高度表达。需要进一步研究以揭示类胰蛋白酶在 BMI 增加与 HOA 病理之间关系中的作用。
