National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Cooperative Innovation Center of Sustainable Pig Production, Wuhan 430070, China.
Int J Mol Sci. 2023 Jul 24;24(14):11842. doi: 10.3390/ijms241411842.
(APP) is the causative pathogen of porcine pleuropneumonia, a highly contagious respiratory disease in the pig industry. The increasingly severe antimicrobial resistance in APP urgently requires novel antibacterial alternatives for the treatment of APP infection. In this study, we investigated the effect of tea polyphenols (TP) against APP. MIC and MBC of TP showed significant inhibitory effects on bacteria growth and caused cellular damage to APP. Furthermore, TP decreased adherent activity of APP to the newborn pig tracheal epithelial cells (NPTr) and the destruction of the tight adherence junction proteins β-catenin and occludin. Moreover, TP improved the survival rate of APP infected mice but also attenuated the release of the inflammation-related cytokines IL-6, IL-8, and TNF-α. TP inhibited activation of the TLR/MAPK/PKC-MLCK signaling for down-regulated TLR-2, TLR4, p-JNK, p-p38, p-PKC-α, and MLCK in cells triggered by APP. Collectively, our data suggest that TP represents a promising therapeutic agent in the treatment of APP infection.
（APP）是猪传染性胸膜肺炎的病原体，是猪养殖业中一种高度传染性的呼吸道疾病。APP 中日益严重的抗微生物药物耐药性迫切需要新型抗菌替代品来治疗 APP 感染。在本研究中，我们研究了茶多酚（TP）对 APP 的作用。TP 的 MIC 和 MBC 对细菌生长表现出显著的抑制作用，并导致 APP 细胞损伤。此外，TP 降低了 APP 对新生猪气管上皮细胞（NPTr）的黏附活性，并破坏了紧密连接蛋白β-连环蛋白和闭合蛋白。此外，TP 提高了 APP 感染小鼠的存活率，同时减轻了与炎症相关的细胞因子 IL-6、IL-8 和 TNF-α的释放。TP 抑制了 TLR/MAPK/PKC-MLCK 信号通路的激活，从而下调了 APP 触发细胞中 TLR-2、TLR4、p-JNK、p-p38、p-PKC-α 和 MLCK。总的来说，我们的数据表明，TP 是治疗 APP 感染的一种有前途的治疗剂。
