Complex Structure Surgical Sciences and Technologies, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.
Spine Surgery Unit, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.
Int J Mol Sci. 2023 Jul 24;24(14):11856. doi: 10.3390/ijms241411856.
Recently, our group described the application of vertebral bone marrow (vBMA) clot as a cell therapy strategy for spinal fusion. Its beneficial effects were confirmed in aging-associated processes, but the influence of gender is unknown. In this study, we compared the biological properties of vBMA clots and derived vertebral mesenchymal stem cells (MSCs) from female and male patients undergoing spinal fusion procedures and treated with vBMA clot. We analyzed the expression of growth factors (GFs) in vBMA clots and MSCs as well as morphology, viability, doubling time, markers expression, clonogenicity, differentiation ability, senescence factors, Klotho expression, and HOX and TALE gene profiles from female and male donors. Our findings indicate that vBMA clots and derived MSCs from males had higher expression of GFs and greater osteogenic and chondrogenic potential compared to female patients. Additionally, vBMA-clot-derived MSCs from female and male donors exhibited distinct levels of HOX and TALE gene expression. Specifically, HOXA1, HOXB8, HOXD9, HOXA11, and PBX1 genes were upregulated in MSCs derived from clotted vBMA from male donors. These results demonstrate that vBMA clots can be effectively used for spinal fusion procedures; however, gender-related differences should be taken into consideration when utilizing vBMA-clot-based studies to optimize the design and implementation of this cell therapy strategy in clinical trials.
最近，我们小组描述了使用椎骨骨髓（vBMA）凝块作为脊柱融合细胞治疗策略的应用。其在与年龄相关的过程中的有益效果已得到证实，但性别影响尚不清楚。在这项研究中，我们比较了接受脊柱融合手术并接受 vBMA 凝块治疗的女性和男性患者的 vBMA 凝块和衍生的椎骨间充质干细胞（MSCs）的生物学特性。我们分析了 vBMA 凝块和 MSCs 中生长因子（GFs）的表达以及形态、活力、倍增时间、标志物表达、克隆形成能力、分化能力、衰老因子、Klotho 表达以及来自女性和男性供体的 HOX 和 TALE 基因谱。我们的研究结果表明，与女性患者相比，来自男性的 vBMA 凝块和衍生的 MSCs 具有更高的 GFs 表达和更强的成骨和成软骨潜力。此外，来自女性和男性供体的 vBMA 凝块衍生的 MSCs 表现出不同水平的 HOX 和 TALE 基因表达。具体而言，HOXA1、HOXB8、HOXD9、HOXA11 和 PBX1 基因在来自男性供体的凝块 vBMA 衍生的 MSC 中上调。这些结果表明，vBMA 凝块可有效用于脊柱融合手术；然而，在使用基于 vBMA 凝块的研究时，应考虑性别相关差异，以优化这种细胞治疗策略在临床试验中的设计和实施。
