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使用结扎的椎骨骨髓促进脊柱手术后组织再生的基本原理。

A Rationale for the Use of Clotted Vertebral Bone Marrow to Aid Tissue Regeneration Following Spinal Surgery.

机构信息

Laboratory of Preclinical and Surgical Studies, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.

Department of Oncological and Degenerative Spine Surgery, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.

出版信息

Sci Rep. 2020 Mar 5;10(1):4115. doi: 10.1038/s41598-020-60934-2.

DOI:10.1038/s41598-020-60934-2
PMID:32139727
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7058026/
Abstract

Vertebral body bone marrow aspirate (V-BMA), easily accessible simultaneously with the preparation of the site for pedicle screw insertion during spinal procedures, is becoming an increasingly used cell therapy approach in spinal surgery. However, the main drawbacks for V-BMA use are the lack of a standardized procedure and of a structural texture with the possibility of diffusion away from the implant site. The aim of this study was to evaluate, characterize and compare the biological characteristics of MSCs from clotted V-BMA and MSCs from whole and concentrate V-BMAs. MSCs from clotted V-BMA showed the highest cell viability and growth factors expression (TGF-β, VEGF-A, FGF2), the greatest colony forming unit (CFU) potency, cellular homogeneity, ability to differentiate towards the osteogenic (COL1AI, TNFRSF11B, BGLAP) and chondrogenic phenotype (SOX9) and the lowest ability to differentiate toward the adipogenic lineage (ADIPOQ) in comparison to all the other culture conditions. Additionally, results revealed that MSCs, differently isolated, expressed different level of HOX and TALE signatures and that PBX1 and MEIS3 were down-regulated in MSCs from clotted V-BMA in comparison to concentrated one. The study demonstrated for the first time that the cellular source inside the clotted V-BMA showed the best biological properties, representing an alternative and advanced cell therapy approach for patients undergoing spinal surgery.

摘要

椎体骨髓抽吸（V-BMA），在脊柱手术中，可在准备椎弓根螺钉插入部位的同时进行，它作为一种细胞治疗方法，在脊柱外科中应用越来越广泛。然而，V-BMA 应用的主要缺点是缺乏标准化的程序和结构质地，存在从植入部位扩散的可能性。本研究旨在评估、表征和比较凝结的 V-BMA 中的间充质干细胞（MSCs）和全血及浓缩的 V-BMA 中的 MSCs 的生物学特性。与其他所有培养条件相比，凝结的 V-BMA 中的 MSCs 具有最高的细胞活力和生长因子表达（TGF-β、VEGF-A、FGF2）、最大的集落形成单位（CFU）潜能、细胞均一性、向成骨（COL1AI、TNFRSF11B、BGLAP）和软骨形成表型（SOX9）分化的能力，以及向脂肪形成谱系（ADIPOQ）分化的能力最低。此外，结果表明，不同分离的 MSCs 表达不同水平的 HOX 和 TALE 特征，与浓缩的 V-BMA 中的 MSCs 相比，凝结的 V-BMA 中的 PBX1 和 MEIS3 下调。该研究首次证明，凝结的 V-BMA 中的细胞来源具有最佳的生物学特性，为接受脊柱手术的患者提供了一种替代和先进的细胞治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/a3105d64f7fd/41598_2020_60934_Fig11_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/d88ad52beeb2/41598_2020_60934_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/a3105d64f7fd/41598_2020_60934_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/d4ac38fbb305/41598_2020_60934_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/bc642fec04a4/41598_2020_60934_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/37df154f3a28/41598_2020_60934_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/6fe0e5a4d631/41598_2020_60934_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/c6fbe74a33f7/41598_2020_60934_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/31f2ad125173/41598_2020_60934_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/2434d35d24e4/41598_2020_60934_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/0f06b48ee148/41598_2020_60934_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/2e9993a98c63/41598_2020_60934_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/d88ad52beeb2/41598_2020_60934_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1d/7058026/a3105d64f7fd/41598_2020_60934_Fig11_HTML.jpg

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