Mashkilleyson N, Eskelinen A
Z Hautkr. 1986 Jul 1;61(13):975-81.
A scanning absorbance cytophotometry of nuclear DNA in the cells of Malpighian layer was made of biopsies obtained from the perilesional areas of 23 patients with different severity of pemphigus vulgaris. The quantity of DNA was expressed in relative units of ploidy with regard to peripheral blood lymphocyte diploid nuclei. The ratio of cells with diploid to tetraploid DNA-values in the Malpighian layer of 30 controls was 71.4 +/- 2.5% to 24.6 +/- 2.7% (p less than 0.05). A statistically significant greater number of cells with tetraploid DNA content and a corresponding lower number of those with the diploid value of DNA was revealed in all the examined patients with pemphigus vulgaris. These changes were most pronounced in the group of seven patients in whom the course of the disease was particularly severe. In these patients the mean ratio of the diploid to tetraploid DNA nuclei was 39.2 +/- 0.8% to 54.6 +/- 2.3% (p less than 0.01).
对23例不同严重程度寻常型天疱疮患者病损周围区域的活检组织进行了马尔皮基层细胞核DNA的扫描吸收细胞光度测定。DNA数量以相对于外周血淋巴细胞二倍体细胞核的倍性相对单位表示。30名对照者马尔皮基层中二倍体与四倍体DNA值细胞的比例为71.4±2.5%至24.6±2.7%（p<0.05）。在所有接受检查的寻常型天疱疮患者中，均发现具有四倍体DNA含量的细胞数量在统计学上显著增多，而具有二倍体DNA值的细胞数量相应减少。这些变化在7例病情特别严重的患者组中最为明显。在这些患者中，二倍体与四倍体DNA细胞核的平均比例为39.2±0.8%至54.6±2.3%（p<0.01）。
